Biphasic calcium phosphate macroporous scaffolds derived from oyster shells for bone tissue engineering

► A new biphasic calcium phosphate macroporous scaffold for bone tissue engineering was fabricated. ► Oyster shell waste as raw material. ► An innovative combination of hydrothermal reaction and polymer replication method. ► The scaffolds were characterized by high porosity and excellent permeabilit...

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Bibliographic Details
Published in:Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2011-10, Vol.173 (3), p.837-845
Main Authors: Yang, Yun, Yao, Qingqing, Pu, Ximing, Hou, Zhenqing, Zhang, Qiqing
Format: Article
Language:English
Subjects:
Applied sciences
biocompatibility
Calcium phosphate
Chemical engineering
Crassostrea angulata
Crystallization, leaching, miscellaneous separations
Exact sciences and technology
Hydrothermal reaction
hydroxyapatite
macropores
micropores
nanocrystals
permeability
polymers
porosity
Porous scaffold
powders
raw materials
Replication method
shell (molluscs)
Tissue engineering
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Summary:► A new biphasic calcium phosphate macroporous scaffold for bone tissue engineering was fabricated. ► Oyster shell waste as raw material. ► An innovative combination of hydrothermal reaction and polymer replication method. ► The scaffolds were characterized by high porosity and excellent permeability. ► The scaffolds had excellent in vitro biocompatibility with pre-osteoblasts. The aim of this work was to fabricate a macroporous scaffold from oyster shell by using an innovative combination of techniques. Crassostrea angulata shell characterized by a natural microporous structure (2–10 μm) was used as raw material. Firstly, plate-like nanocrystals of AB-type carbonated hydroxyapatite (HA) were produced by hydrothermal conversion of fine-milled oyster shell powders. Secondly, interconnected macroporous scaffolds were prepared with the polymer replication method. The obtained scaffolds presented a biphasic structure of hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) with a porosity of 91.4 ± 1.2%, and showed an excellent permeability due to the open macropores (200–500 μm) and interconnected micropores (100–500 nm) in macropore walls. The synthetic scaffolds were found to be non-cytotoxic and displayed better biocompatibility than pure HA scaffolds when seeded with pre-osteoblasts cells (MC3T3-E1). Therefore, the macroporous scaffolds derived from oyster shells would offer promising alternatives for bone tissue engineering applications.
ISSN:1385-8947
1873-3212
DOI:10.1016/j.cej.2011.07.029