Anti-inflammatory effects of resveratrol occur via inhibition of lipopolysaccharide-induced NF-κB activation in Caco-2 and SW480 human colon cancer cells

Resveratrol, a polyphenol abundantly found in grapes and red wine, exhibits beneficial health effects due to its anti-inflammatory properties. In the present study, we evaluated the effect of resveratrol on inflammatory responses induced by lipopolysaccharide (LPS) treatment of human intestinal Caco...

Full description

Saved in:
Bibliographic Details
Published in:British journal of nutrition 2012-11, Vol.108 (9), p.1623-1632
Main Authors: Panaro, Maria Antonietta, Carofiglio, Vito, Acquafredda, Angela, Cavallo, Pasqua, Cianciulli, Antonia
Format: Article
Language:English
Subjects:
Anti-Inflammatory Agents, Non-Steroidal - adverse effects
Anti-Inflammatory Agents, Non-Steroidal - metabolism
Anti-Inflammatory Agents, Non-Steroidal - therapeutic use
Antineoplastic Agents, Phytogenic - adverse effects
Antineoplastic Agents, Phytogenic - metabolism
Antineoplastic Agents, Phytogenic - therapeutic use
Biological and medical sciences
Caco-2 Cells
Cell Line, Tumor
Cell Survival
Colonic Neoplasms - immunology
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Colonic Neoplasms - prevention & control
Dietary Supplements - adverse effects
Down-Regulation
Enterocytes - cytology
Enterocytes - immunology
Enterocytes - metabolism
Enterocytes - pathology
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Humans
I-kappa B Proteins - metabolism
Inflammatory Bowel Diseases - diet therapy
Inflammatory Bowel Diseases - immunology
Inflammatory Bowel Diseases - metabolism
Inflammatory Bowel Diseases - pathology
Lipopolysaccharides
NF-kappa B - antagonists & inhibitors
NF-kappa B - metabolism
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - genetics
Nitric Oxide Synthase Type II - metabolism
Nutritional Immunology
Phosphorylation
Protein Processing, Post-Translational
Proteolysis
RNA, Messenger - metabolism
Stilbenes - adverse effects
Stilbenes - metabolism
Stilbenes - therapeutic use
Toll-Like Receptor 4 - genetics
Toll-Like Receptor 4 - metabolism
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Resveratrol, a polyphenol abundantly found in grapes and red wine, exhibits beneficial health effects due to its anti-inflammatory properties. In the present study, we evaluated the effect of resveratrol on inflammatory responses induced by lipopolysaccharide (LPS) treatment of human intestinal Caco-2 and SW480 cell lines. In the LPS-treated intestinal cells, resveratrol dose-dependently inhibited the expression of inducible NO synthase (iNOS) mRNA as well as protein expression, resulting in a decreased production of NO. In addition, Toll-like receptor-4 expression was significantly diminished in LPS-stimulated cells after resveratrol pre-treatment. To investigate the mechanisms by which resveratrol reduces NO production and iNOS expression, we examined the activation of inhibitor of κB (IκB) in LPS-stimulated intestinal cells. Results demonstrated that resveratrol inhibited the phosphorylation, as well as the degradation, of the IκB complex. Overall, these results show that resveratrol is able to reduce LPS-induced inflammatory responses by intestinal cells, interfering with the activation of NF-κB-dependent molecular mechanisms.
ISSN:0007-1145
1475-2662
DOI:10.1017/S0007114511007227