Loading…
Modification of E. coli central metabolism to optimize the biotransformation of L-isoleucine into 4-hydroxyisoleucine by enzymatic hydroxylation
The enzymes of the Entner-Doudoroff pathway (EDP) and/or the oxidative branch of the pentose phosphate pathway (OBPPP) have been inactivated in the E. coli 2Δ (Δ sucAB , Δ aceAK , P L - brnQ ) [pEL-IDO] strain, which is used for the biotransformation of L-isoleucine into 4-hydroxyisoleucine (4-HIL)...
Saved in:
Published in: | Applied biochemistry and microbiology 2012-12, Vol.48 (7), p.639-644 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | The enzymes of the Entner-Doudoroff pathway (EDP) and/or the oxidative branch of the pentose phosphate pathway (OBPPP) have been inactivated in the
E. coli
2Δ (Δ
sucAB
, Δ
aceAK
, P
L
-
brnQ
) [pEL-IDO] strain, which is used for the biotransformation of L-isoleucine into 4-hydroxyisoleucine (4-HIL) by enzymatic hydroxylation. We established that the inactivation of the EDP in the 2Δ [pEL-IDO] strain does not virtually affect the basic parameters of the process, whereas the blocking of the OBPPP reduces the efficiency of biomass accumulation and L-isoleucine transformation in conversion to glucose. However, the simultaneous inactivation of the enzymes of the EDP and OBPPP in the 2Δ [pEL-IDO] strain allowed us to achieve a considerable improvement of the process, namely, a 20% reduction in glucose consumption and a 35% decrease in biomass yield at a constant L-isoleucine/4-HIL conversion rate at a level of 88%. |
---|---|
ISSN: | 0003-6838 1608-3024 |
DOI: | 10.1134/S0003683812070034 |