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The function of mitochondrial FOF1 ATP-synthase from the whiteleg shrimp Litopenaeus vannamei muscle during hypoxia
The effect of hypoxia and re-oxygenation on the mitochondrial complex FOF1-ATP synthase was investigated in the whiteleg shrimp Litopenaeus vannamei. A 660kDa protein complex isolated from mitochondria of the shrimp muscle was identified as the ATP synthase complex. After 10h at hypoxia (1.5–2.0mg o...
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Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2012-08, Vol.162 (4), p.107-112 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The effect of hypoxia and re-oxygenation on the mitochondrial complex FOF1-ATP synthase was investigated in the whiteleg shrimp Litopenaeus vannamei. A 660kDa protein complex isolated from mitochondria of the shrimp muscle was identified as the ATP synthase complex. After 10h at hypoxia (1.5–2.0mg oxygen/L), the concentration of L-lactate in plasma increased significantly, but the ATP amount and the concentration of ATPβ protein remained unaffected. Nevertheless, an increase of 70% in the ATPase activity was detected, suggesting that the enzyme may be regulated at a post-translational level. Thus, during hypoxia shrimp are able to maintain ATP amounts probably by using some other energy sources as phosphoarginine when an acute lack of energy occurs. During re-oxygenation, the ATPase activity decreased significantly and the ATP production continued via the electron transport chain and oxidative phosphorylation. The results obtained showed that shrimp faces hypoxia partially by hydrolyzing the ATP through the reaction catalyzed by the mitochondrial ATPase which increases its activity. |
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ISSN: | 1096-4959 1879-1107 |
DOI: | 10.1016/j.cbpb.2012.03.004 |