Electrochemical detection of Salmonella using gold nanoparticles

A disposable immunosensor for Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S) detection using a magneto-immunoassay and gold nanoparticles (AuNPs) as label for electrochemical detection is developed. The immunosensor is based on the use of a screen-printed carbon electrode (SPCE) tha...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2013-02, Vol.40 (1), p.121-126
Main Authors: Afonso, André S., Pérez-López, Briza, Faria, Ronaldo C., Mattoso, Luiz H.C., Hernández-Herrero, Manuela, Roig-Sagués, Artur Xavier, Maltez-da Costa, Marisa, Merkoçi, Arben
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
Cattle
Conductometry - instrumentation
Electrochemical detection
Equipment Design
Equipment Failure Analysis
Food Analysis - instrumentation
Food Contamination - analysis
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Gold
Gold nanoparticles
Hygiene and safety
Immunomagnetic Separation - instrumentation
Label
Magneto-immunoassay
Metal Nanoparticles
Milk - microbiology
Reproducibility of Results
Salmonella
Salmonella enterica - isolation & purification
Sensitivity and Specificity
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Summary:A disposable immunosensor for Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S) detection using a magneto-immunoassay and gold nanoparticles (AuNPs) as label for electrochemical detection is developed. The immunosensor is based on the use of a screen-printed carbon electrode (SPCE) that incorporates a permanent magnet underneath. Salmonella containing samples (i.e. skimmed milk) have been tested by using anti-Salmonella magnetic beads (MBs-pSAb) as capture phase and sandwiching afterwards with AuNPs modified antibodies (sSAb-AuNPs) detected using differential pulse voltammetry (DPV). A detection limit of 143 cellsmL−1 and a linear range from 103 to 106 cellsmL−1 of Salmonella was obtained, with a coefficient of variation of about 2.4%. Recoveries of the sensor by spiking skimmed milk with different quantities of Salmonella of about 83% and 94% for 1.5×103 and 1.5×105 cellsmL−1 were obtained, respectively. This AuNPs detection technology combined with magnetic field application reports a limit of detection lower than the conventional commercial method carried out for comparison purposes in skimmed milk samples Rapid Salmonella detection by using gold nanoparticles. [Display omitted]
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2012.06.054