Mapping of crown gall resistance locus Rcg1 in grapevine

Agrobacteria are efficient plant pathogens. They are able to transform plant cells genetically resulting in abnormal cell proliferation. Cultivars of Vitis vinifera are highly susceptible to many virulent Agrobacterium strains but certain wild Vitis species, including Vitis amurensis have resistant...

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Bibliographic Details
Published in:Theoretical and applied genetics 2012-11, Vol.125 (7), p.1565-1574
Main Authors: Kuczmog, Anett, Galambos, Anikó, Horváth, Szabina, Mátai, Anikó, Kozma, Pál, Szegedi, Ernő, Putnoky, Péter
Format: Article
Language:English
Subjects:
Agriculture
Agrobacterium
Agrobacterium - physiology
Base Sequence
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell growth
Chromosome mapping
Chromosome Mapping - methods
Chromosome Segregation - genetics
Chromosomes, Plant - genetics
Cloning
Crown-gall disease
Cultivars
Development and progression
Disease Resistance - genetics
Diseases and pests
Flowers & plants
Genes, Plant - genetics
Genetic aspects
Genetic Loci - genetics
Genetic Markers
Genetic Testing
Genomes
Genomics
Grapes
Life Sciences
Localization
Microsatellite Repeats - genetics
Original Paper
Pathogens
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Diseases - genetics
Plant Diseases - immunology
Plant Diseases - microbiology
Plant Genetics and Genomics
Plant immunology
Plant Tumors - genetics
Plant Tumors - microbiology
Random Amplified Polymorphic DNA Technique
Recombination, Genetic
Vitaceae
Vitis
Vitis - genetics
Vitis - immunology
Vitis - microbiology
Vitis vinifera
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Summary:Agrobacteria are efficient plant pathogens. They are able to transform plant cells genetically resulting in abnormal cell proliferation. Cultivars of Vitis vinifera are highly susceptible to many virulent Agrobacterium strains but certain wild Vitis species, including Vitis amurensis have resistant genotypes. Studies of the molecular background of such natural resistance are of special importance, not only for practical benefits in agricultural practice but also for understanding the role of plant genes in the transformation process. Earlier, crown gall resistance from V. amurensis was introgressed into V. vinifera through interspecific breeding and it was shown to be inherited as a single and dominant Mendelian trait. To develop this research further, towards understanding underlying molecular mechanisms, a mapping population was established, and resistance-coupled molecular DNA markers were identified by three different approaches. First, RAPD makers linked to the resistance locus ( Rcg1 ) were identified, and on the basis of their DNA sequences, we developed resistance-coupled SCAR markers. However, localization of these markers in the grapevine genome sequence failed due to their similarity to many repetitive regions. Next, using SSR markers of the grapevine reference linkage map, location of the resistance locus was established on linkage group 15 (LG15). Finally, this position was supported further by developing new chromosome-specific markers and by the construction of the genetic map of the region including nine loci in 29.1 cM. Our results show that the closest marker is located 3.3 cM from the Rcg1 locus that may correspond to 576 kb.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-012-1935-2