Microsatellite Mixed-Stock Identification of Coho Salmon in British Columbia

Variation at 17 microsatellite loci was analyzed for about 50,000 coho salmon Oncorhynchus kisutch sampled from 274 locations ranging from Russia to California (but largely from British Columbia), and the variation was applied to estimate stock composition in mixed-stock fishery samples. High resolu...

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Bibliographic Details
Published in:Marine and coastal fisheries 2012-01, Vol.4 (1), p.85-100
Main Authors: Beacham, Terry D, Candy, John R, Wallace, Colin, Wetklo, Michael, Deng, Langtuo, MacConnachie, Cathy
Format: Article
Language:English
Subjects:
Accuracy
Alleles
Composition
Deoxyribonucleic acid
DNA
Estimates
Fish hatcheries
Fisheries
Fishing
Freshwater fishes
Genetic testing
Identification
Laboratories
Local population
Marine
Microsatellites
Migrations
Oncorhynchus kisutch
Population
Population genetics
Regions
Rivers
Salmon
Stock identification
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Description
Summary:Variation at 17 microsatellite loci was analyzed for about 50,000 coho salmon Oncorhynchus kisutch sampled from 274 locations ranging from Russia to California (but largely from British Columbia), and the variation was applied to estimate stock composition in mixed-stock fishery samples. High resolution of mixed-stock samples was possible; accurate estimates of stock composition were available for coho salmon originating from 39 regions (Russia, 1 region; Yukon River, 1; southeast Alaska, 1; British Columbia, 28; Washington, 5; Columbia River, 1; Oregon, 1; California, 1). The power of a locus in providing accurate estimates of stock composition of simulated single-population mixtures was related to the number of alleles observed at the locus. Approximately 800 alleles were observed across the 17 microsatellites. Analysis of known-origin samples indicated that accurate regional estimates of stock composition were obtained; estimates from 37 of 39 regions had accuracy greater than 90%. Estimated stock compositions of five mixed-fishery samples collected in British Columbia and the San Juan Islands (Washington) reflected the presence and timing of migration of the local populations. Microsatellites provided accurate estimates of stock composition from many locations in the British Columbia distribution of coho salmon.
ISSN:1942-5120
1942-5120
DOI:10.1080/19425120.2012.661393