First identification of Flavobacterium columnare infection in farmed freshwater striped catfish Pangasianodon hypophthalmus

The bacterium Flavobacterium columnare was recovered and identified as the aetiological agent causing freshwater columnaris infection in farmed striped catfish Pangasianodon hypophthalmus (Sauvage) fingerlings that had suffered high mortality rates within commercial hatchery ponds in Vietnam. The gr...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2012-08, Vol.100 (1), p.83-88
Main Authors: Tien, Nguyen Thi, Dung, Tu Thanh, Tuan, Nguyen Anh, Crumlish, Mags
Format: Article
Language:English
Subjects:
Animals
Aquaculture
Catfishes - microbiology
Disease Outbreaks - veterinary
Fish Diseases - epidemiology
Fish Diseases - microbiology
Fish Diseases - pathology
Flavobacteriaceae Infections - epidemiology
Flavobacteriaceae Infections - microbiology
Flavobacteriaceae Infections - pathology
Flavobacteriaceae Infections - veterinary
Flavobacterium - classification
Flavobacterium - isolation & purification
Flavobacterium columnare
Freshwater
Hypophthalmus
Marine
Pangasianodon hypophthalmus
Time Factors
Vietnam - epidemiology
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Summary:The bacterium Flavobacterium columnare was recovered and identified as the aetiological agent causing freshwater columnaris infection in farmed striped catfish Pangasianodon hypophthalmus (Sauvage) fingerlings that had suffered high mortality rates within commercial hatchery ponds in Vietnam. The gross clinical signs were typical of columnaris-infected fish. Histological examination found numerous Gram-negative, filamentous bacteria present on the skin, muscle and gill tissues of affected fish. The yellow-pigmented bacteria were isolated and identified as F. columnare using primary, biochemical and PCR methods. An experimental immersion-challenge study with 2 strains was also performed. It fulfilled Koch's postulates and showed a median lethal concentration (LC50) of 4.27 × 105 and 1.66 × 106 cfu ml-1 for the F. columnare strains FC-HN and FC-CT, respectively. To the best of our knowledge this is the first report of freshwater columnaris infection in P. hypophthalmus.
ISSN:0177-5103
0171-8630
1616-1580
DOI:10.3354/dao02478