Analysis of the Major Metabolite of Delta D9-Tetrahydrocannabinol in Urine II. A HPLC Procedure

A high performance liquid chromatographic (HPLC) procedure was developed for the analysis of 11-nor- Delta *D9-tetrahydrocannabinol-9-carboxylic acid in urine. Hydrolyzed urine samples were cleaned up using Bond-Elut? columns. 11-Nor-cannabinol-9-carboxylic acid was used as internal standard. The an...

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Bibliographic Details
Published in:Journal of analytical toxicology 1983-11, Vol.7 (6), p.262-264
Main Authors: Elsohly, Mahmoud A, Elsohly, Hala N, Jones, Alan B, Dimson, Philip A, Wells, Kirk E
Format: Article
Language:English
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Summary:A high performance liquid chromatographic (HPLC) procedure was developed for the analysis of 11-nor- Delta *D9-tetrahydrocannabinol-9-carboxylic acid in urine. Hydrolyzed urine samples were cleaned up using Bond-Elut? columns. 11-Nor-cannabinol-9-carboxylic acid was used as internal standard. The analysis was carried out using a C8 reversed-phase column with acetonitrile:50 mM phosphoric acid (65:35) as the solvent. Detection was done by ultraviolet detector at 214 nm. The minimum detectable level of 11-nor- Delta *D9-tetrahydrocannabinol-9-carboxylic acid was 25 ng/mL.
ISSN:0146-4760