Evaluation of thiol-modified hyaluronan and elastin-like polypeptide composite augmentation in early-stage disc degeneration: comparing 2 minimally invasive techniques

An in vitro biomechanical and imaging study generated from an in vivo porcine model of early stage degenerative disc disease was used to evaluate mechanical property restoration, comparing 2 minimally invasive injection techniques. To evaluate the ability of an injectable hydrogel to restore the mec...

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Bibliographic Details
Published in:Spine (Philadelphia, Pa. 1976) Pa. 1976), 2012-09, Vol.37 (20), p.E1296-E1303
Main Authors: Leckie, Ashley E, Akens, Margarete K, Woodhouse, Kimberly A, Yee, Albert J M, Whyne, Cari M
Format: Article
Language:English
Subjects:
Animals
Biomechanical Phenomena
Disease Models, Animal
Elastin - chemistry
Humans
Hyaluronic Acid - chemistry
Hyaluronic Acid - pharmacology
Hydrogel, Polyethylene Glycol Dimethacrylate
Intervertebral Disc - drug effects
Intervertebral Disc - physiopathology
Intervertebral Disc - surgery
Intervertebral Disc Degeneration - physiopathology
Intervertebral Disc Degeneration - therapy
Lumbar Vertebrae - drug effects
Lumbar Vertebrae - physiopathology
Lumbar Vertebrae - surgery
Minimally Invasive Surgical Procedures - methods
Peptides - chemistry
Peptides - pharmacology
Range of Motion, Articular
Sulfhydryl Compounds - chemistry
Swine
Time Factors
Tissue Engineering - methods
Total Disc Replacement - methods
Treatment Outcome
Viscosupplements - pharmacology
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Summary:An in vitro biomechanical and imaging study generated from an in vivo porcine model of early stage degenerative disc disease was used to evaluate mechanical property restoration, comparing 2 minimally invasive injection techniques. To evaluate the ability of an injectable hydrogel to restore the mechanical properties of spinal motion segments with early stage disc degeneration, comparing 2 minimally invasive injection techniques. Treatment of early-stage disc degeneration may benefit from a combination of tissue engineering and minimally invasive therapeutic approaches. A recently developed hydrogel, thiol-modified hyaluronan elastin-like polypeptide (TMHA/EP) composite, has demonstrated potential as an injectable nucleus replacement. From a total of thirteen 35-kg Yorkshire boars, early-stage lumbar disc degeneration was introduced into 10 pigs via injection of chondroitinase ABC. After degeneration, 8 pigs received TMHA/EP augmentation; 1 disc via direct needle injection and a second using a modified kyphoplasty approach. High-resolution magnetic resonance images were acquired of the excised spinal motion segments, followed by biomechanical testing in axial compression, flexion-extension, lateral bending, and torsion. The degenerate control motion segments were generally less stiff and more flexible than healthy controls. The injection of TMHA/EP into the degenerated nucleus produced similar mechanical stiffness to healthy controls. The direct-injected discs showed a dispersive pattern of TMHA/EP within the nucleus, whereas the modified kyphoplasty method yielded a bolus of hydrogel. Yet, mechanical behavior was comparable considering the 2 minimally invasive augmentation techniques. The TMHA/EP composite can restore initial mechanical behavior in early-stage disc degeneration. Although both augmentation methods yielded mechanical properties comparable with healthy controls, direct injection represents a simpler technique, uses a smaller-gauge needle, does not introduce air into the disc, and yields a dispersive pattern that may be beneficial for future delivery of cells or growth factors.
ISSN:0362-2436
1528-1159
DOI:10.1097/BRS.0b013e318266ecea