Differential loss of heterozygosity profile on chromosome 3p in ductal and lobular breast carcinomas

Summary The 2 main histologic types of infiltrating breast cancer, invasive lobular and invasive ductal carcinoma, are morphologically and clinically distinct. Studies revealed that different patterns of gene expression and loss of heterozygosity can also distinguish these 2 subtypes. A whole-genome...

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Bibliographic Details
Published in:Human pathology 2012-10, Vol.43 (10), p.1661-1667
Main Authors: de Oliveira, Márcia Maria Costa, MSc, de Oliveira, Sarah Franco Vieira, MSc, Lima, Rubens Silveira, MD, de Andrade Urban, Cícero, MD, PhD, Cavalli, Luciane Regina, PhD, de Souza Fonseca Ribeiro, Enilze Maria, MD, PhD, Cavalli, Iglenir João, PhD
Format: Article
Language:English
Subjects:
Biological and medical sciences
Breast cancer
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Carcinoma, Ductal, Breast - genetics
Carcinoma, Ductal, Breast - pathology
Carcinoma, Lobular - genetics
Carcinoma, Lobular - pathology
Chromosomes, Human, Pair 3 - genetics
Ductal breast carcinoma
Female
Genetics
Gynecology. Andrology. Obstetrics
Humans
Investigative techniques, diagnostic techniques (general aspects)
Lobular breast carcinoma
LOH
Loss of Heterozygosity - genetics
Mammary gland diseases
Medical sciences
Mutation
Pathology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Polymerase Chain Reaction
Proteins
Tumors
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Summary:Summary The 2 main histologic types of infiltrating breast cancer, invasive lobular and invasive ductal carcinoma, are morphologically and clinically distinct. Studies revealed that different patterns of gene expression and loss of heterozygosity can also distinguish these 2 subtypes. A whole-genome study using single nucleotide polymorphism array found a significantly higher frequency of loss of heterozygosity on 3p in invasive ductal carcinoma when compared with invasive lobular carcinoma. In this study, we performed a loss of heterozygosity analysis of the 3p chromosome region in ductal and lobular breast tumors. Seven microsatellite markers were evaluated in a series of 136 sporadic breast cancer cases (118 invasive ductal carcinoma and 18 invasive lobular carcinoma) and correlated with clinical-histopathologic parameters from the patients. A significantly higher frequency of loss of heterozygosity was observed in invasive ductal carcinoma (65.3%) when compared with invasive lobular carcinoma (38.9%). When the markers were analyzed separately, loss of heterozygosity at 3 of them, D3S1307 in 3p26.3, D3S1286 in 3p24.3, and D3S1300 in 3p14.2, were significantly more frequent in ductal than in lobular tumors. D3S1307 marker showed the highest frequency of loss of heterozygosity in invasive ductal carcinoma (46.2%), and associations between loss of this marker and loss of estrogen and progesterone receptors were found in these samples. Our results confirm the observations that invasive ductal carcinoma has a higher frequency of loss of heterozygosity events across the 3p region than invasive lobular carcinoma and show that specific losses on 3p26.3, 3p24.3, and 3p14.2 regions are more frequent in ductal than in lobular tumors. We discuss our data in relation to the known tumor suppressor genes that are mapped at the 3p loci investigated and their present relevant roles in breast cancer.
ISSN:0046-8177
1532-8392
DOI:10.1016/j.humpath.2011.12.008