Quality of Sperm Obtained by Penile Vibratory Stimulation and Percutaneous Vasal Sperm Aspiration in Men With Spinal Cord Injury

: The aim of this study was to explore sperm chromosomal aneuploidy and DNA integrity in infertile patients with spinal cord injury (SCI). Semensamples were collected from 12 infertile menwith SCI by percutaneous vasal sperm aspiration (PVSA) and from 14 male SCI patients by penile vibratory stimula...

Full description

Saved in:
Bibliographic Details
Published in:Journal of andrology 2012-09, Vol.33 (5), p.1036-1046
Main Authors: Qiu, Yi, Wang, Lei‐Guang, Zhang, Li‐Hong, Zhang, Ai‐Dong, Wang, Zhong‐Ye
Format: Article
Language:English
Subjects:
Adult
anejaculation
Aneuploidy
Biological and medical sciences
Birth control
Case-Control Studies
Cell Shape
Chi-Square Distribution
Chromosome aberrations
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 21
Chromosomes, Human, X
Chromosomes, Human, Y
DNA Fragmentation
Fundamental and applied biological sciences. Psychology
Gynecology. Andrology. Obstetrics
Humans
In Situ Hybridization, Fluorescence
In Situ Nick-End Labeling
Infertility, Male - etiology
Infertility, Male - genetics
Infertility, Male - pathology
Infertility, Male - physiopathology
Male
Male genital diseases
male infertility
Mammalian male genital system
Medical genetics
Medical sciences
Penis - innervation
SCI
Semen Analysis
sperm DNA fragmentation
Sperm Motility
Sperm Retrieval
Spermatozoa - pathology
Spinal Cord Injuries - complications
Spinal Cord Injuries - genetics
Spinal Cord Injuries - pathology
Spinal Cord Injuries - physiopathology
Sterility. Assisted procreation
Suction
Vertebrates: reproduction
Vibration
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: The aim of this study was to explore sperm chromosomal aneuploidy and DNA integrity in infertile patients with spinal cord injury (SCI). Semensamples were collected from 12 infertile menwith SCI by percutaneous vasal sperm aspiration (PVSA) and from 14 male SCI patients by penile vibratory stimulation (PVS). These semen samples as well as samples from 16 donors were analyzed using the hypo‐osmotic swelling (HOS) test, the sperm chromatin dispersion test, terminal deoxynucleotidyl transferase‐mediated terminal uridine nick‐end labeling assay, and multicolor fluorescence in situ hybridization with probes specific for the chromosomes 13, 18, 21, X, and Y. There were significant differences in the percentages of motile sperm, normal morphologic sperm, normal HOS/eosin staining, and sperm DNA fragmentation between the infertile men with SCI and the control group (P < .05 and P < .01). The sperm forward motility was significantly greater in the PVSA group than in the PVS group (P < .01). The number of round cells per milliliter of semen obtained from the 14 SCI patients by PVS was between 1 million and 12 million. The rate of sperm DNA fragmentation, as identified by the sperm chromatin dispersion test, was higher in the PVS group than in the PVSA group (P < .05). The aneuploidy rates for the SCI patients were 1.5‐ to 1.6‐fold higher for chromosomes 13, 18, and 21, and were 2.3‐ to 2.4‐fold higher for chromosomes X and Y than for patients in the control group (P < .001). These results suggest that for men with SCI, the semen quality is poorer, the prevalence of abnormal HOS/eosin staining is greater, and sperm DNA fragmentation and sperm chromosomal aneuploidies are seen at a higher rate compared with healthy, fertile, and normospermic men.
ISSN:0196-3635
1939-4640
DOI:10.2164/jandrol.111.014902