Stability, oviposition attraction, and larvicidal activity of binary toxin from Bacillus sphaericus expressed in Escherichia coli

Bacillus sphaericus produces a two-chain binary toxin composed of BinA (42 kDa) and BinB (51 kDa), which are deposited as parasporal crystals during sporulation. The toxin is highly active against Culex larvae and Aedes and Anopheles mosquitoes, which are the principal vectors for the transmission o...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2012-09, Vol.95 (5), p.1235-1241
Main Authors: da Silva Pinto, Luciano, Gonçales, Relber Aguiar, Conceição, Fabricio Rochedo, Knabah, Paula Ferreira, Borsuk, Sibele, Campos, Vinicius Farias, Arruda, Francisco Vassiliepe, Leite, Fabio Pereira Leivas
Format: Article
Language:English
Subjects:
Aedes
Animals
Anopheles
Bacillus sphaericus
Bacillus thuringiensis
Bacillus thuringiensis - genetics
Bacterial Toxins - genetics
Bacterial Toxins - isolation & purification
Bacterial Toxins - toxicity
Biological control
Biomedical and Life Sciences
Biotechnologically Relevant Enzymes and Proteins
Biotechnology
Bottled water industry
Chemotactic Factors - isolation & purification
Chemotactic Factors - pharmacology
Cloning
Culex
Culex - drug effects
Culex quinquefasciatus
E coli
Encephalitis
Escherichia coli
Escherichia coli - genetics
Gene Expression
Insect control
Insecticides - isolation & purification
Insecticides - pharmacology
Larva - drug effects
Life Sciences
Malaria
Microbial Genetics and Genomics
Microbiology
Mosquitoes
Oviposition - drug effects
Pests
Proteins
Recombinant proteins
Recombinant Proteins - genetics
Recombinant Proteins - isolation & purification
Recombinant Proteins - toxicity
Sedimentation & deposition
Studies
Survival Analysis
Toxicity
Tropical diseases
Yellow fever
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Summary:Bacillus sphaericus produces a two-chain binary toxin composed of BinA (42 kDa) and BinB (51 kDa), which are deposited as parasporal crystals during sporulation. The toxin is highly active against Culex larvae and Aedes and Anopheles mosquitoes, which are the principal vectors for the transmission of malaria, yellow fever, encephalitis, and dengue. The use of B. sphaericus and Bacillus thuringiensis in mosquito control programs is limited by their sedimentation in still water. In this study, the binA and binB genes were cloned and the recombinant BinAB protein was expressed in three strains of Escherichia coli . These recombinant strains were used in a toxicity assay against Culex quinquefasciatus larvae. The highest expression level was achieved when both proteins were expressed in a single operon construct. The BinAB protein expressed in the E. coli Arctic strain showed higher larvicidal activity than either of the recombinant proteins from the E. coli Ril or pLysS strains. Furthermore, it had the highest oviposition attraction (49.1%, P  
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-011-3808-z