A gold nanoparticles-modified aptamer beacon for urinary adenosine detection based on structure-switching/fluorescence-“turning on” mechanism

A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3′-en...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2012-11, Vol.70, p.362-368
Main Authors: Zhang, Jin-Quan, Wang, Yong-Sheng, Xue, Jin-Hua, He, Yan, Yang, Hui-Xian, Liang, Jun, Shi, Lin-Fei, Xiao, Xi-Lin
Format: Article
Language:English
Subjects:
Adenosine
Adenosine - chemistry
Adenosine - urine
Analysis
Analytical, structural and metabolic biochemistry
Aptamer beacon
Aptamers, Nucleotide - chemistry
Biological and medical sciences
Biosensing Techniques - standards
Calibration
Chromatography, High Pressure Liquid
detection limit
fluorescence
Fluorescence Resonance Energy Transfer - standards
Fluorescence-“turning on”
Fundamental and applied biological sciences. Psychology
General pharmacology
Gold
Gold nanoparticles
high performance liquid chromatography
Humans
Hydrogen-Ion Concentration
Limit of Detection
Medical sciences
Metal Nanoparticles
Molecular Probe Techniques - standards
nucleotide sequences
oligonucleotides
Pharmacology. Drug treatments
quantitative analysis
Reference Standards
Sensitivity and Specificity
Spectrometry, Fluorescence
Spectroscopy, Fourier Transform Infrared
Structure-switching
Temperature
Time Factors
Tromethamine - chemistry
Urinalysis
urine
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Description
Summary:A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis. The Ade aptamer was engineered into an aptamer beacon by adding a gold nanoparticle-modified nucleotide sequence which is complementary to aptamer sequence (FDNA) at the 3′-end of FDNA. The fluorescence signal “turning on” was observed when AB was bound to Ade, which is attributed to a significant conformational change in AB from a FDNA/QDNA duplex to a FDNA-Ade complex. The Ade measurement was carried out in 20mmolL−1 Tris–HCl buffer solution of pH 7.4, ΔF signal linearly correlated with the concentration of Ade over the range of 2.0×10−8 to 1.8×10−6molL−1. The limit of detection (LOD) for Ade is 6.0×10−9molL−1 with relative standard deviations (R.S.D) of 3.64–5.36%, and the recoveries were 98.6%, 100%, 102% (n=6), respectively. The present method has been successfully applied to determine Ade in human urine samples, and the obtained results were in good agreement with those obtained by the HPLC method. Our investigation shows that the unique properties of the AB could provide a promising potential for small molecules detection, and be benefit to extend the application of aptamer beacon technique.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2012.05.032