Identification of Baiying (Herba Solani Lyrati) commodity and its toxic substitute Xungufeng (Herba Aristolochiae Mollissimae) using DNA barcoding and chemical profiling techniques

► Baiying (Solanum lyratum) was adulterated by Xungufeng (Aristolochia mollissima). ► Molecular identification by DNA barcodes (ITS, matK, rbcL, trnH-psbA and trnL-trnF). ► Chemical identification by TLC and HPLC using aristolochic acids as markers. ► Biological cytotoxicity assay on HEK-293 and Ver...

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Bibliographic Details
Published in:Food chemistry 2012-12, Vol.135 (3), p.1653-1658
Main Authors: Li, Ming, Au, Ka-Yee, Lam, Hilary, Cheng, Ling, Jiang, Ren-Wang, But, Paul Pui-Hay, Shaw, Pang-Chui
Format: Article
Language:English
Subjects:
acids
adulterated products
Animals
Aristolochia
Aristolochia - chemistry
Aristolochia - classification
Aristolochia - genetics
Aristolochia mollissima
Aristolochic acid
Aristolochic acids
Authentication
Biological and medical sciences
carcinogenicity
Cercopithecus aethiops
cytotoxicity
DNA barcoding
DNA Barcoding, Taxonomic
Drug Contamination
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - standards
Drugs, Chinese Herbal - toxicity
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
HEK293 Cells
herbs
high performance liquid chromatography
Humans
markets
Methods of analysis, processing and quality control, regulation, standards
Molecular Sequence Data
nucleotide sequences
Plant Proteins - genetics
Quality Control
Solanum - chemistry
Solanum - classification
Solanum - genetics
Solanum lyratum
thin layer chromatography
traditional medicine
Vero Cells
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Summary:► Baiying (Solanum lyratum) was adulterated by Xungufeng (Aristolochia mollissima). ► Molecular identification by DNA barcodes (ITS, matK, rbcL, trnH-psbA and trnL-trnF). ► Chemical identification by TLC and HPLC using aristolochic acids as markers. ► Biological cytotoxicity assay on HEK-293 and Vero kidney cell lines. ► An integrated molecular, chemical and biological approach for better quality control. Baiying derived from Solanum lyratum Hance is a commonly consumed natural product for ethnomedical treatment of cancer. One of the substitutes present in the market is a carcinogenic aristolochic acids-containing herb Xungufeng derived from Aristolochia mollissima Thunb. The purpose of this study is to establish DNA barcodes, thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and cytotoxicity assay to differentiate Baiying from Xungufeng. A total of 30 DNA sequences from five DNA barcodes (ITS, matK, rbcL, trnH-psbA and trnL-trnF) were generated to differentiate S. lyratum from A. mollissima and authenticate ten samples of Baiying and Xungufeng commodities. Using aristolochic acids as standard markers, TLC and HPLC analyses also successfully authenticated these commodities. In vitro cytotoxicity assay using HEK-293 and Vero cells demonstrated that Xungufeng was significantly more toxic than Baiying. This is the first study applying an integrated molecular, chemical and biological approach to differentiate traditional Chinese medicine from Aristolochia adulterant.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2012.06.049