Intraspecific diversity of Edwardsiella ictaluri isolates from diseased freshwater catfish, Pangasianodon hypophthalmus (Sauvage), cultured in the Mekong Delta, Vietnam

A molecular epidemiology study was conducted on 90 Edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, Pangasianodon hypophthalmus, cultured in the Mekong Delta, Vietnam. Thirteen isolates of E. ictaluri derived from diseased channel catfish, Ictalurus punctatus, cultur...

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Bibliographic Details
Published in:Journal of fish diseases 2012-09, Vol.35 (9), p.671-682
Main Authors: Bartie, K L, Austin, F W, Diab, A, Dickson, C, Dung, T T, Giacomini, M, Crumlish, M
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Bacterial Typing Techniques
Brackish
Catfishes
DNA Restriction Enzymes - metabolism
Edwardsiella ictaluri
Edwardsiella ictaluri - classification
Edwardsiella ictaluri - drug effects
Edwardsiella ictaluri - genetics
Edwardsiella ictaluri - isolation & purification
Enterobacteriaceae Infections - microbiology
Enterobacteriaceae Infections - veterinary
Fish Diseases - microbiology
Fresh Water
Freshwater
Genetic Variation
genotyping
Ictaluridae - microbiology
Ictalurus punctatus
Microbial Sensitivity Tests
Pangasianodon hypophthalmus
Phylogeny
Plasmids - genetics
pulsed-field gel electrophoresis
Rep-PCR
Species Specificity
United States
Vietnam
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Summary:A molecular epidemiology study was conducted on 90 Edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, Pangasianodon hypophthalmus, cultured in the Mekong Delta, Vietnam. Thirteen isolates of E. ictaluri derived from diseased channel catfish, Ictalurus punctatus, cultured in the USA were included for comparison. All the E.  ictaluri isolates tested were found to be biochemically indistinguishable. A repetitive (rep)‐PCR using the single (GTG)5 primer was shown to possess limited discriminatory power, yielding two similar DNA profiles categorized as (GTG)5‐PCR group 1 or 2 among the Vietnam isolates and (GTG)5‐PCR group 1 within the USA isolates. Macrorestriction analysis identified 14 and 22 unique pulsotypes by XbaI and SpeI, respectively, among a subset of 59 E. ictaluri isolates. Numerical analysis of the combined macrorestriction profiles revealed three main groups: a distinct cluster formed exclusively of the USA isolates, and a major and minor cluster with outliers contained the Vietnam isolates. Antibiotic susceptibility and plasmid profiling supported the existence of the three groups. The results indicate that macrorestriction analysis may be regarded as a suitable typing method among the E. ictaluri species of limited intraspecific diversity. Furthermore, the findings suggest that E. ictaluri originating from Vietnam may constitute a distinct genetic group.
ISSN:0140-7775
1365-2761
DOI:10.1111/j.1365-2761.2012.01376.x