Targeting CreER(T2) expression to keratin 8-expressing murine simple epithelia using bacterial artificial chromosome transgenesis

Keratin 8 (K8) is a type II keratin that is associated with the type I keratins K18 or K19 in single layered epithelia. We generated a bacterial artificial chromosome (BAC) transgenic mouse line that expresses the tamoxifen inducible CreER(T2) inserted into the endogenous murine K8 gene. The transge...

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Bibliographic Details
Published in:Transgenic research 2012-10, Vol.21 (5), p.1117-1123
Main Authors: Zhang, Li, Zhang, Boyu, Han, Sang Jun, Shore, Amy N, Rosen, Jeffrey M, Demayo, Francesco J, Xin, Li
Format: Article
Language:English
Subjects:
Animals
Chromosomes, Artificial, Bacterial - metabolism
Enzyme Activation
Epithelial Cells - cytology
Epithelial Cells - metabolism
Female
Gene Expression Regulation
Gene Targeting - methods
Gene Transfer Techniques
Genes, Reporter
Genotyping Techniques
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Immunohistochemistry
Integrases - genetics
Integrases - metabolism
Keratin-8 - genetics
Keratin-8 - metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Fluorescence
Tamoxifen - metabolism
Tamoxifen - pharmacology
Transgenes
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Summary:Keratin 8 (K8) is a type II keratin that is associated with the type I keratins K18 or K19 in single layered epithelia. We generated a bacterial artificial chromosome (BAC) transgenic mouse line that expresses the tamoxifen inducible CreER(T2) inserted into the endogenous murine K8 gene. The transgenic mouse line contains two copies of the BAC transgene. To determine the expression specificity and inducibility of CreER(T2), the K8-CreER(T2) mice were bred with a Gt(ROSA 26)( ACTB-tdTomato-EGFP ) fluorescent protein-based reporter transgenic mouse line. We demonstrated that CreER(T2) and the endogenous K8 gene share the same patterns of expression and that the enzymatic activity of CreER(T2) can be efficiently induced by tamoxifen in all K8-expressing tissues. This mouse line will be useful for studying gene function in development and homeostasis of simple epithelia, and investigating both tissue lineage hierarchy and the identity of the cells of origin for epithelial cancers.
ISSN:1573-9368
DOI:10.1007/s11248-012-9598-y