Water avoidance stress results in an altered voiding phenotype in male mice

Aims We set out to characterize the voiding phenotypes of male mice to a water avoidance stress (WAS) protocol and compare the molecular changes with those induced by surgically induced partial bladder outlet obstruction (pBOO). Methods Six‐week‐old male Swiss Webster mice housed with sibling litter...

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Bibliographic Details
Published in:Neurourology and urodynamics 2012-09, Vol.31 (7), p.1185-1189
Main Authors: McGonagle, Erin, Smith, Ariana, Butler, Stephan, Sliwoski, Joanna, Valentino, Rita, Canning, Douglas, Zderic, Stephen A.
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus
Animals
Behavior, Animal
Disease Models, Animal
Electrophoretic Mobility Shift Assay
HIF
Hypertrophy
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
Male
Mice
Myosin Heavy Chains - genetics
Myosin Heavy Chains - metabolism
NFATC Transcription Factors - metabolism
outlet obstruction
Phenotype
Polymerase Chain Reaction
stress
Stress, Psychological - complications
Stress, Psychological - genetics
Stress, Psychological - metabolism
Stress, Psychological - physiopathology
Stress, Psychological - psychology
Time Factors
Urinary Bladder - metabolism
Urinary Bladder - pathology
Urinary Bladder - physiopathology
Urinary Bladder Neck Obstruction - complications
Urinary Bladder Neck Obstruction - genetics
Urinary Bladder Neck Obstruction - metabolism
Urinary Bladder Neck Obstruction - physiopathology
Urination
Urination Disorders - etiology
Urination Disorders - genetics
Urination Disorders - metabolism
Urination Disorders - physiopathology
Urination Disorders - psychology
Urodynamics
voiding dysfunction
Water
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Summary:Aims We set out to characterize the voiding phenotypes of male mice to a water avoidance stress (WAS) protocol and compare the molecular changes with those induced by surgically induced partial bladder outlet obstruction (pBOO). Methods Six‐week‐old male Swiss Webster mice housed with sibling littermates were individually placed on a platform centered in the middle of a water filled basin for 1 hr daily for 4 weeks. A non stressed cohort of sibling littermates served as controls. Measured end points included voiding frequency, voided volume, bladder mass, and in vivo cystometry. Molecular end points included myosin heavy chain (MHC) isoform distribution by PCR, and nuclear translocation of hypoxia inducible factor (HIF1α) and the nuclear factor of activated T‐cells (NFAT) by gel shift assay. These molecular endpoints were compared with samples from male mice undergoing anatomic pBOO. Results WAS resulted in increased average voided volumes and bladder mass, and a decrease in voiding frequency (P 
ISSN:0733-2467
1520-6777
DOI:10.1002/nau.22207