Isolation and characteristics of eight novel polymorphic microsatellite loci in Lippia alba (Verbenaceae)

• Premise of the study: A set of eight microsatellite (simple sequence repeat [SSR]) markers for Lippia alba, an important medicinal and cosmetic plant, was developed to aid studies of genetic diversity and to define efficient strategies for breeding programs. • Methods and Results: Using a (CT)8‐ a...

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Bibliographic Details
Published in:American journal of botany 2012-08, Vol.99 (8), p.e301-e303
Main Authors: Santos, Fernanda R. C., Lima, Paula F., Priolli, Regina H. G., Siqueira, Walter J., Colombo, Carlos A.
Format: Article
Language:English
Subjects:
Alleles
Base Sequence
Botany
DNA Primers - genetics
DNA, Plant - genetics
essential oil
Flowers & plants
Gene Library
Gene loci
Genetic diversity
Genetic Loci
Genetic Markers
Lippia - classification
Lippia - genetics
Lippia alba
medicinal plant
Microsatellite Repeats - genetics
Molecular Sequence Data
Plant Leaves - classification
Plant Leaves - genetics
Plants, Medicinal
Polymorphism
Polymorphism, Genetic
Sequence Analysis, DNA
simple sequence repeat markers
Species Specificity
Verbenaceae
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Summary:• Premise of the study: A set of eight microsatellite (simple sequence repeat [SSR]) markers for Lippia alba, an important medicinal and cosmetic plant, was developed to aid studies of genetic diversity and to define efficient strategies for breeding programs. • Methods and Results: Using a (CT)8‐ and (GT)8‐enriched library, a total of 11 SSR loci were developed and optimized in L. alba. Of the 11 loci, eight were found to be polymorphic after screening 61 accessions from two populations. The parameters used to characterize loci were expected heterozygosity (He) and number of alleles. A total of 44 alleles were identified, with an average of 5.5 alleles per loci, which were moderately to highly informative according to He. • Conclusions: These new SSR markers have potential for informing genetic diversity, allele mining, and mapping studies and will be used to generate information for breeding programs of L. alba.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1100578