No proinflammatory signature in CD34+ hematopoietic progenitor cells in multiple sclerosis patients

Autologous hematopoietic stem cell transplantation (aHSCT) has been used as a therapeutic approach in multiple sclerosis (MS). However, it is still unclear if the immune system that emerges from autologous CD34+ hematopoietic progenitor cells (HPC) of MS patients is pre-conditioned to re-develop the...

Full description

Saved in:
Bibliographic Details
Published in:Multiple sclerosis 2012-08, Vol.18 (8), p.1188-1192
Main Authors: Lutterotti, A, Jelčić, I, Schulze, C, Schippling, S, Breiden, P, Mazzanti, B, Reinhardt, S, DiGioia, M, Repice, A, Massacesi, L, Sputtek, A, Salinas-Riester, G, Kroeger, N, Sospedra, M, Saccardi, R, Zander, A, Martin, R
Format: Article
Language:English
Subjects:
Antigens, CD34 - analysis
Autografts
Biological and medical sciences
Case-Control Studies
CD34 antigen
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Donors
Female
Gene expression
Gene Expression Profiling - methods
Genomes
Genotype
Hematopoietic Stem Cells - immunology
Hemopoiesis
Humans
Immune system
Inflammation
Inflammation - genetics
Inflammation - immunology
Male
Medical sciences
MicroRNAs - analysis
miRNA
Multiple sclerosis
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Multiple Sclerosis, Chronic Progressive - genetics
Multiple Sclerosis, Chronic Progressive - immunology
Multiple Sclerosis, Relapsing-Remitting - genetics
Multiple Sclerosis, Relapsing-Remitting - immunology
Neurology
Peripheral blood
Phenotype
Principal Component Analysis
Statistics
stem cell transplantation
Stem cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Autologous hematopoietic stem cell transplantation (aHSCT) has been used as a therapeutic approach in multiple sclerosis (MS). However, it is still unclear if the immune system that emerges from autologous CD34+ hematopoietic progenitor cells (HPC) of MS patients is pre-conditioned to re-develop the proinflammatory phenotype. The objective of this article is to compare the whole genome gene and microRNA expression signature in CD34+ HPC of MS patients and healthy donors (HD). CD34+ HPC were isolated from peripheral blood of eight MS patients and five HD and analyzed by whole genome gene expression and microRNA expression microarray. Among the differentially expressed genes (DEGs) only TNNT1 reached statistical significance (logFC=3.1, p
ISSN:1352-4585
1477-0970
DOI:10.1177/1352458511434067