The multiplicity of Staphylococcus aureus in chronic rhinosinusitis: Correlating surface biofilm and intracellular residence

Objectives/Hypothesis: The biofilm paradigm of chronic rhinosinusitis (CRS) is increasingly understood to play a key role in the pathophysiology of this disease. The role of intracellular infection of sinonasal epithelial cells has been suggested as a potential reservoir of pathogenic organisms that...

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Bibliographic Details
Published in:The Laryngoscope 2012-08, Vol.122 (8), p.1655-1660
Main Authors: Tan, Neil C.-W., Foreman, Andrew, Jardeleza, Camille, Douglas, Richard, Tran, Hai, Wormald, Peter John
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biofilms
Biological and medical sciences
Chronic Disease
Chronic rhinosinusitis
Cross-Sectional Studies
Endoscopy
Female
Humans
In Situ Hybridization, Fluorescence
intracellular infection
Level of Evidence: 2c
Male
Medical sciences
Microscopy, Confocal
Middle Aged
Nasal Mucosa - microbiology
Non tumoral diseases
Otorhinolaryngology (head neck, general aspects and miscellaneous)
Otorhinolaryngology. Stomatology
Prospective Studies
Rhinitis - epidemiology
Rhinitis - microbiology
Rhinitis - surgery
Sinusitis - epidemiology
Sinusitis - microbiology
Sinusitis - surgery
Staphylococcal Infections - epidemiology
Staphylococcal Infections - microbiology
Staphylococcal Infections - surgery
Staphylococcus aureus
Staphylococcus aureus - physiology
Statistics as Topic
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Objectives/Hypothesis: The biofilm paradigm of chronic rhinosinusitis (CRS) is increasingly understood to play a key role in the pathophysiology of this disease. The role of intracellular infection of sinonasal epithelial cells has been suggested as a potential reservoir of pathogenic organisms that can lead to recalcitrant disease despite maximal medical and surgical treatment. Could a surface biofilm play a role in allowing intracellular infection to occur, and what are the factors associated with potential intracellular infections? The aim of this study was to investigate these questions. Study Design: A prospective study including 36 CRS patients undergoing endoscopic sinus surgery and five control patients undergoing endonasal pituitary surgery. Methods: Sinonasal mucosa harvested at the time of surgery was examined with a Staphylococcus aureus fluorescence in situ hybridization probe and propodium iodide counterstain using the confocal scanning laser microscope for both biofilm status and evidence of intracellular organisms. Results: Intracellular S aureus was identified in 20/36 (56%) CRS patients compared to 0/8 (0%) control patients. CRS patients with intracellular infection were significantly more likely to harbor surface biofilm (20/20, P = .0014) and have a S aureus‐positive culture swab (12/20, P = .0485). Conclusions: This study gives further evidence supporting a role of intracellular S aureus in CRS. In all cases intracellular infection was associated with surface biofilm, suggesting a potential relationship between the two. Further work is required to delineate the true mechanisms of intracellular persistence and also the role that it plays in the recalcitrant nature of CRS. Laryngoscope, 2012
ISSN:0023-852X
1531-4995
DOI:10.1002/lary.23317