Myosin-activating protein kinases are possible regulators of nonmuscle myosin in developing human heart

We studied the localization of myosin-activating protein kinases in cardiomyocytes obtained from fetal human heart at 8–9 weeks gestation. It was found that at this developmental stage, smooth muscle/nonmuscle myosin light chain kinase (MLCK, 108 kDa) and its high-molecular weight isoform (MLCK, 210...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2011-12, Vol.152 (2), p.198-201, Article 198
Main Authors: Stepanova, O. V., Chadin, A. V., Masyutin, A. G., Kulikova, T. G., Poltavceva, R. A., Masenko, V. P., Sukhikh, G. T.
Format: Article
Language:eng ; rus
Subjects:
Apoptosis Regulatory Proteins - metabolism
Biomedical and Life Sciences
Biomedicine
Biophysics and Biochemistry
Calcium-Calmodulin-Dependent Protein Kinases - metabolism
Cell Biology
Cells, Cultured
Death-Associated Protein Kinases
Female
Heart - embryology
Humans
Internal Medicine
Laboratory Medicine
Muscle proteins
Myocytes, Cardiac - metabolism
Myofibrils - metabolism
Myosin
Myosin-Light-Chain Kinase - metabolism
Myosins - metabolism
Pathology
Pregnancy
Protein kinases
Sarcomeres - metabolism
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Summary:We studied the localization of myosin-activating protein kinases in cardiomyocytes obtained from fetal human heart at 8–9 weeks gestation. It was found that at this developmental stage, smooth muscle/nonmuscle myosin light chain kinase (MLCK, 108 kDa) and its high-molecular weight isoform (MLCK, 210 kDa), skeletal MLCK and death-associated protein kinase (DAPK) are co-localized with nonmuscle myosin IIB in the premyofibrils. The data obtained suggest that cardiac nonmuscle myosin at 8–9 weeks gestation may serve as the substrate of the studied myosin-activating protein kinases that are likely to cooperatively regulate the formation of myofibrils. We revealed high-molecular weight isoform of smooth muscle/nonmuscle kinase MLCK-210 in developing human heart and determined the ratios of MLCK-108 and MLCK-210 at different gestational stages. In this case, the approximate time period of changes in these isoforms ratio was revealed (between 8–9 and 13 weeks), that can be associated with functional changes in the developing myocardium.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-011-1487-5