Adsorption of ochratoxin A and zearalenone by potential probiotic Saccharomyces cerevisiae strains and its relation with cell wall thickness

Aims:  To examine Saccharomyces cerevisae strains with previously reported beneficial properties and aflatoxin B1 binding capacity, for their ability to remove ochratoxin A (OTA) and zearalenone (ZEA) and to study the relation between cell wall thickness and detoxificant ability of yeast strains. Me...

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Bibliographic Details
Published in:Journal of applied microbiology 2012-08, Vol.113 (2), p.256-264
Main Authors: Armando, M.R., Pizzolitto, R.P., Dogi, C.A., Cristofolini, A., Merkis, C., Poloni, V., Dalcero, A.M., Cavaglieri, L.R.
Format: Article
Language:English
Subjects:
Adsorption
Aflatoxin B1 - metabolism
Animal Feed
Bile - chemistry
Biological and medical sciences
Cell Wall - ultrastructure
cell wall thickness
Cellular biology
Fundamental and applied biological sciences. Psychology
Gastric Juice - chemistry
gastrointestinal conditions
Hydrogen-Ion Concentration
Microbiology
ochratoxin A
Ochratoxins - metabolism
Probiotics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae - ultrastructure
Yeast
zearalenone
Zearalenone - metabolism
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Summary:Aims:  To examine Saccharomyces cerevisae strains with previously reported beneficial properties and aflatoxin B1 binding capacity, for their ability to remove ochratoxin A (OTA) and zearalenone (ZEA) and to study the relation between cell wall thickness and detoxificant ability of yeast strains. Methods and Results:  A mycotoxin binding assay at different toxin concentrations and the effect of gastrointestinal conditions on mycotoxin binding were evaluated. Ultrastructural studies of yeast cells were carried out with transmission electronic microscopy. All tested strains were capable of removing OTA and ZEA. Saccharomyces cerevisiae RC012 and RC016 showed the highest OTA removal percentage, whereas RC009 and RC012 strains showed the highest ZEA removal percentages. The cell diameter/cell wall thickness relation showed a correlation between cell wall amount and mycotoxin removal ability. After exposure to gastrointestinal conditions, a significant increase in mycotoxin binding was observed. Conclusions:  All tested Saccharomyces cerevisiae strains were able to remove OTA and ZEA, and physical adsorption would be the main mechanism involved in ochratoxin A and ZEA removal. Gastrointestinal conditions would enhance adsorption and not decrease mycotoxin–adsorbent interactions. Significance and Impact of the Study:  Live strains with mycotoxin binding ability and beneficial properties are potential probiotics that could be included in animal feed. Previous and present results suggest that the RC008 and RC016 strains are very promising candidates for functional feed product development.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2012.05331.x