Sirtuin1 in tracheal aspirate leukocytes: possible role in the development of bronchopulmonary dysplasia in premature infants

Objective: To study the association between Sirtuin1 (Sirt1), a class III histone deacetylator, in tracheal aspirate (TA) leukocytes and the development of bronchopulmonary dysplasia (BPD) in premature infants and modulation of Sirt1 with dexamethasone (Dex) use. Design/methods: Serial TA samples we...

Full description

Saved in:
Bibliographic Details
Published in:The journal of maternal-fetal & neonatal medicine 2012-08, Vol.25 (8), p.1483-1487
Main Authors: Mody, Kartik, Saslow, Judy G., Kathiravan, Suganya, Eydelman, Riva, Bhat, Vishwanath, Stahl, Gary E., Pyon, Kee, Bhandari, Vineet, Aghai, Zubair H.
Format: Article
Language:English
Subjects:
Biopsy, Needle
Bronchopulmonary Dysplasia - drug therapy
Bronchopulmonary Dysplasia - etiology
Bronchopulmonary Dysplasia - mortality
Bronchopulmonary Dysplasia - pathology
CLD
Cohort Studies
Dexamethasone - therapeutic use
Female
Gestational Age
HDAC
Humans
Infant Mortality
Infant, Newborn
Infant, Premature - metabolism
Leukocytes - chemistry
Leukocytes - metabolism
Leukocytes - pathology
lung
Male
neonate
Sirtuin 1 - analysis
Sirtuin 1 - metabolism
Sirtuin 1 - physiology
Trachea - chemistry
Trachea - metabolism
Trachea - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: To study the association between Sirtuin1 (Sirt1), a class III histone deacetylator, in tracheal aspirate (TA) leukocytes and the development of bronchopulmonary dysplasia (BPD) in premature infants and modulation of Sirt1 with dexamethasone (Dex) use. Design/methods: Serial TA samples were collected on days 1, 3, 5 and 7 from ventilated premature neonates. Sirt1 was localized by immunocytochemistry and quantified on a scale of 0-4 by blinded observers. BPD was defined as the need of supplemental oxygen at 36 weeks postmenstrual age (PMA). Results: A total of 130 TA samples were collected from 51 infants (mean ± SD: GA 25.5 ± 1.4 w, BW 762 ± 174 g). Eleven infants survived without BPD and 40 infants died before 36 weeks PMA or developed BPD. Sirt1 was localized in the cytoplasm and nuclei of mononuclear (MONO) as well as polymorphonuclear cells. Sirt1 was significantly more localized in the nuclei of MONO cells in infants without BPD compared to infants who developed BPD or died before 36 weeks PMA. Twenty six infants received Dex. There was no significant change in Sirt1 localization with steroid therapy. Conclusions: Lower Sirt1 in TA leukocytes is associated with the development of BPD or death in premature infants. Dex use had no effect on Sirt1.
ISSN:1476-7058
1476-4954
DOI:10.3109/14767058.2011.645925