Stage-specific changes in calcium concentration in crustacean (Callinectes sapidus) Y-organs during a natural molting cycle, and their relation to the hemolymphatic ecdysteroid titer

Secretion of ecdysteroid molting hormones by crustacean Y-organs is suppressed by molt-inhibiting hormone (MIH). The suppressive effect of MIH on ecdysteroidogenesis is mediated by one or more cyclic nucleotide second messengers. In addition, existing data indicate that ecdysteroidogenesis is positi...

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Published in:Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2012-09, Vol.163 (1), p.170-173
Main Authors: Chen, Hsiang-Yin, Dillaman, Richard M., Roer, Robert D., Watson, R. Douglas
Format: Article
Language:English
Subjects:
Animals
Brachyura - anatomy & histology
Brachyura - metabolism
Ca++ signaling
calcium
Calcium - metabolism
Callinectes sapidus
crabs
Crustacean
cyclic nucleotides
ecdysteroidogenesis
Ecdysteroids
Ecdysteroids - metabolism
Endocrine Glands - cytology
Endocrine Glands - metabolism
fluorescence
hemolymph
Molting
radioimmunoassays
second messengers
secretion
Y-organ
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Summary:Secretion of ecdysteroid molting hormones by crustacean Y-organs is suppressed by molt-inhibiting hormone (MIH). The suppressive effect of MIH on ecdysteroidogenesis is mediated by one or more cyclic nucleotide second messengers. In addition, existing data indicate that ecdysteroidogenesis is positively regulated (stimulated) by intracellular Ca++. Despite the apparent critical role of calcium in regulating ecdysteroidogenesis, the level of Ca++ in Y-organ cells has not been previously measured during a natural molting cycle for any crustacean species. In studies reported here, a fluorescent calcium indicator (Fluo-4) was used to measure Ca++ levels in Y-organs during a molting cycle of the blue crab, Callinectes sapidus. Mean calcium fluorescence increased 5.8-fold between intermolt (C4) and stage D3 of premolt, and then dropped abruptly, reaching a level in postmolt (A) that was not significantly different from that in intermolt (P>0.05). The level of ecdysteroids in hemolymph of Y-organ donor crabs (measured by radioimmunoassay) showed an overall pattern similar to that observed for calcium fluorescence, rising from 2.9ng/mL in intermolt to 357.1ng/mL in D3 (P
ISSN:1095-6433
1531-4332
DOI:10.1016/j.cbpa.2012.05.205