Facile Preparation of Zwitterion-Stabilized Superparamagnetic Iron Oxide Nanoparticles (ZSPIONs) as an MR Contrast Agent for in Vivo Applications

We describe a simple method for synthesizing superparamagnetic nanoparticles (SPIONs) as small, stable contrast agents for magnetic resonance imaging (MRI) based on sulfobetaine zwitterionic ligands. SPIONs synthesized by thermal decomposition were coated with zwitterions to impart water dispersibil...

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Bibliographic Details
Published in:Langmuir 2012-06, Vol.28 (25), p.9634-9639
Main Authors: Kim, Dongkyu, Chae, Min Kyung, Joo, Hyun Jung, Jeong, Il-ha, Cho, Jee-Hyun, Lee, Chulhyun
Format: Article
Language:English
Subjects:
Animals
Carcinoma, Lewis Lung - diagnosis
Cell Line, Tumor
Chemistry
Colloidal state and disperse state
Contrast Media - chemistry
Exact sciences and technology
Ferric Compounds - chemistry
General and physical chemistry
Magnetic Resonance Imaging - methods
Magnets - chemistry
Mice
Mice, Inbred BALB C
Nanoparticles - chemistry
Oleic Acid - chemistry
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Surface-Active Agents - chemistry
Water - chemistry
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Summary:We describe a simple method for synthesizing superparamagnetic nanoparticles (SPIONs) as small, stable contrast agents for magnetic resonance imaging (MRI) based on sulfobetaine zwitterionic ligands. SPIONs synthesized by thermal decomposition were coated with zwitterions to impart water dispersibility and high in vivo stability through the nanoemulsion method. Zwitterion surfactant coating layers are formed easily on oleic acid-stabilized SPIONs via hydrophobic and van der Waals interactions. Our zwitterion-coated SPIONs (ZSPIONs) had ultrathin (∼5 nm) coating layers with mean sizes of 12.0 ± 2.5 nm, as measured by dynamic light scattering (DLS). Upon incubation in 1 M NaCl and 10% FBS, the ZSPIONs showed high colloidal stabilities without precipitating, as monitored by DLS. The T2 relaxivity coefficient of the ZSPIONs, obtained by measuring the relaxation rate on the basis of the iron concentration, was 261 mM–1 s–1. This value was much higher than that of the commercial T2 contrast agent because of the ultrathin coating layer. Furthermore, we confirmed that ZSPIONs can be used as MR contrast agents for in vivo applications such as tumor imaging and lymph node mapping.
ISSN:0743-7463
1520-5827
DOI:10.1021/la300043m