Arterial Stiffness Is Associated With Increased Monocyte Expression of Adiponectin Receptor mRNA and Protein in Patients With Coronary Artery Disease

Background Arterial stiffness and carotid intima-media thickness (IMT) constitute validated cardiovascular prognostic markers. Adiponectin and its receptors 1 (AdipoR1) and 2 (AdipoR2) are involved in coronary artery disease (CAD). We investigated whether AdipoR1 and R2 mRNA and protein expression a...

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Published in:American journal of hypertension 2012-07, Vol.25 (7), p.746-755
Main Authors: Ikonomidis, Ignatios, Kadoglou, Nikolaos, Tsiotra, Panayoula C., Kollias, Anastasios, Palios, Ioannis, Fountoulaki, Katerina, Halvatsiotis, Iosif, Maratou, Eirini, Dimitriadis, George, Kremastinos, Dimitrios T., Lekakis, John, Raptis, Sotirios A.
Format: Article
Language:English
Subjects:
Adiponectin - blood
Aged
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Blood and lymphatic vessels
C-Reactive Protein - metabolism
Cardiology. Vascular system
Carotid Intima-Media Thickness
Clinical manifestations. Epidemiology. Investigative techniques. Etiology
Coronary Artery Disease - physiopathology
Cross-Sectional Studies
Diagnostic Techniques, Cardiovascular
Female
Humans
Male
Matrix Metalloproteinase 9 - blood
Medical sciences
Middle Aged
Monocytes - metabolism
Receptors, Adiponectin - genetics
RNA, Messenger - metabolism
Vascular Stiffness
Waist-Hip Ratio
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Summary:Background Arterial stiffness and carotid intima-media thickness (IMT) constitute validated cardiovascular prognostic markers. Adiponectin and its receptors 1 (AdipoR1) and 2 (AdipoR2) are involved in coronary artery disease (CAD). We investigated whether AdipoR1 and R2 mRNA and protein expression are associated with arterial stiffness, IMT and extent of coronary atherosclerosis. Methods We studied 71 patients (61 men, 10 women) with angiographically proven CAD. We measured: (i) monocyte expression of AdipoR1 and AdipoR2 mRNA (quantitative real-time PCR) and protein expression (flow cytometry) (iii) adiponectin, metalloproteinase-9 (MMP-9) and C-reactive protein (CRP) blood levels, (iv) carotid-femoral artery pulse wave velocity (PWV) and carotid IMT. Results Patients with multi-vessel CAD had higher AdipoR1 and AdipoR2 mRNA than those with single-vessel (P < 0.05). PWV was associated with AdipoR1 mRNA (r = 0.474), AdipoR1 protein (r = 0.228), AdipoR2 mRNA (r = 0.716), AdipoR2-protein (r = 0.261), adiponectin (r = 0.236), and MMP-9 (r = 0.350) (P < 0.05, for all correlations). After adjustment for age, sex, waist-hip ratio, and mean blood pressure both AdipoR1 and AdipoR2 mRNA remained independent determinants of PWV (R 2 = 0.35 and R 2 = 0.57, P < 0.05). IMT was also associated with AdipoR2 mRNA, AdipoR2 protein, and MMP-9 (P < 0.05). Increased expression of ADR2 mRNA significantly related to MMP-9 (r = 0.210), and CRP (r = 0.531) (P < 0.05). Conclusion Increased mRNA and protein expression of adiponectin receptors is related with increased aortic stiffness, coronary and peripheral atherosclerosis in patients with CAD. The interrelation of AdipoR2 with inflammatory markers, PWV and IMT suggests a compensatory increase of these receptors to counteract the excess inflammatory and atherogenic process in CAD. Thus, adiponectin receptors may provide a potential therapeutic target of agents activating their beneficial action.
ISSN:0895-7061
1941-7225
1879-1905
DOI:10.1038/ajh.2012.42