Reduction of the monounsaturated fatty acid content of Escherichia coli results in increased resistance to oxidative damage

Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial res...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2012-05, Vol.158 (Pt 5), p.1279-1283
Main Authors: PRADENAS, Gonzalo A, PAILLAVIL, Braulio A, REYES-CERPA, Sebastian, PEREZ-DONOSO, Jose M, VASQUEZ, Claudio C
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Escherichia coli
Escherichia coli - metabolism
Escherichia coli Proteins - metabolism
Fatty Acid Synthase, Type II - metabolism
Fatty Acids, Monounsaturated - metabolism
Fundamental and applied biological sciences. Psychology
Hydro-Lyases - metabolism
Lipid Peroxidation
Microbiology
Miscellaneous
Oxidative Stress
Protein Carbonylation
Reactive Oxygen Species - metabolism
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Summary:Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, fabA). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.056903-0