Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for Solanum elaeagnifolium

Zhu XC, Wu HW, Raman H, Lemerle D, Stanton R & Burrows GE (2012). Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for Solanum elaeagnifolium. Weed Research52, 217–223. Summary Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently...

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Published in:Weed research 2012-06, Vol.52 (3), p.217-223
Main Authors: ZHU, X C, WU, H W, RAMAN, H, LEMERLE, D, STANTON, R, BURROWS, G E
Format: Article
Language:English
Subjects:
Biological and medical sciences
cross-species SSR
Fundamental and applied biological sciences. Psychology
genetic diversity
invasive weed
microsatellite
Parasitic plants. Weeds
Phytopathology. Animal pests. Plant and forest protection
silverleaf nightshade
Weeds
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Summary:Zhu XC, Wu HW, Raman H, Lemerle D, Stanton R & Burrows GE (2012). Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for Solanum elaeagnifolium. Weed Research52, 217–223. Summary Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S. lycopersicum) and eggplant (S. melongena) were tested for cross‐species transferability in S. elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S. elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross‐species SSR markers would be useful for determining the extent of genetic diversity in S. elaeagnifolium populations.
ISSN:0043-1737
1365-3180
DOI:10.1111/j.1365-3180.2012.00908.x