Hydrogels for in vivo-like three-dimensional cellular studies

Extensive efforts have been made to understand the effects of extracellular microenvironments on phenotypic activities for a wide array of stem, progenitor, and precursor cells. Hydrogels have emerged as invaluable platforms for examining the effects of extracellular matrix (ECM) properties on cell...

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Bibliographic Details
Published in:Wiley interdisciplinary reviews. Mechanisms of disease 2012-07, Vol.4 (4), p.351-365
Main Authors: DeVolder, Ross, Kong, Hyun-Joon
Format: Article
Language:English
Subjects:
Alginates - chemistry
Alginates - metabolism
Alginic acid
Biocompatible Materials - chemistry
Biocompatible Materials - metabolism
Biology
Biomedical materials
Cell Adhesion
Cell culture
Cell Culture Techniques
Collagen
Collagen - chemistry
Collagen - metabolism
Controllability
Developmental biology
Drug screening
Extracellular matrix
Extracellular Matrix - chemistry
Extracellular Matrix - metabolism
Fibrin - chemistry
Fibrin - metabolism
Fibrinogen
Glucuronic Acid - chemistry
Glucuronic Acid - metabolism
Hexuronic Acids - chemistry
Hexuronic Acids - metabolism
Hyaluronic Acid - chemistry
Hyaluronic Acid - metabolism
Hydrogels
Hydrogels - chemistry
Hydrogels - metabolism
In vivo methods and tests
Microenvironments
Natural polymers
Polyethylene glycol
Polyethylene Glycols - chemistry
Polymers
Properties (attributes)
Regeneration
Stem cells
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Summary:Extensive efforts have been made to understand the effects of extracellular microenvironments on phenotypic activities for a wide array of stem, progenitor, and precursor cells. Hydrogels have emerged as invaluable platforms for examining the effects of extracellular matrix (ECM) properties on cell activities because of their several advantageous features. Specifically, hydrogels are unique materials that enable cell studies in three‐dimensional (3D) environments, similar to in vivo environments. Recently, there have been increasing efforts to assemble cell‐encapsulating hydrogels; however, hydrogel design strategies for 3D cell cultures have not been systematically discussed to date. Therefore, this review article summarizes current hydrogel designs for 3D cell culture studies and further discusses current challenges and potential resolutions for enhancing the controllability of hydrogel properties and microstructures. The hydrogels discussed herein include those of natural polymers (e.g., collagen, fibrinogen, alginate, and hyaluronic acids), synthetic polymers [e.g., poly(ethylene glycol) (PEG) and its derivatives], and mixtures of natural and synthetic polymers. We envision that hydrogels that enable 3D studies will greatly assist in the understanding of emergent cell behaviors, and ultimately become important biomedical tools for enhancing the quality of in vitro drug screening and clinical treatments. WIREs Syst Biol Med 2012 doi: 10.1002/wsbm.1174 This article is categorized under: Biological Mechanisms > Cell Fates Models of Systems Properties and Processes > Cellular Models Models of Systems Properties and Processes > Organ, Tissue, and Physiological Models Developmental Biology > Stem Cell Biology and Regeneration
ISSN:1939-5094
1939-005X
2692-9368
DOI:10.1002/wsbm.1174