Production and characterization of Clostridium perfringens recombinant β toxoid

In this work, we produced and evaluated a vaccine based on a β toxoid of Clostridium perfringens type C produced in Escherichia coli (rBT). The non-toxic rBT was innocuous for mice and induced 14 IU mL−1 of β antitoxin in rabbits, complying with the European Pharmacopeia and CFR9 - USDA guidelines....

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Bibliographic Details
Published in:Anaerobe 2012-06, Vol.18 (3), p.363-365
Main Authors: Milach, Adalgisa, de los Santos, João Rodrigo Gil, Turnes, Carlos Gil, Moreira, Ângela Nunes, de Assis, Ronnie Antunes, Salvarani, Felipe Masiero, Lobato, Francisco Carlos Faria, Conceição, Fabricio Rochedo
Format: Article
Language:English
Subjects:
Animals
Bacterial Toxins - genetics
Bacterial Vaccines - biosynthesis
Bacterial Vaccines - genetics
Bacterial Vaccines - toxicity
Clostridium perfringens
Clostridium perfringens - immunology
Escherichia coli
Mice
Mice, Inbred BALB C
Rabbits
Recombinant vaccine
Toxoid
Toxoids - biosynthesis
Toxoids - genetics
Toxoids - toxicity
Vaccination
Vaccines, Synthetic - biosynthesis
Vaccines, Synthetic - genetics
Vaccines, Synthetic - toxicity
β toxin
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Summary:In this work, we produced and evaluated a vaccine based on a β toxoid of Clostridium perfringens type C produced in Escherichia coli (rBT). The non-toxic rBT was innocuous for mice and induced 14 IU mL−1 of β antitoxin in rabbits, complying with the European Pharmacopeia and CFR9 - USDA guidelines.
ISSN:1075-9964
1095-8274
DOI:10.1016/j.anaerobe.2012.01.004