protective effect of erdosteine on radiocontrast induced nephrotoxicity in rats

Acute renal failure resulting from radiocontrast-induced nephrotoxicity (RIN) is suggested to occur via medullary ischemia coupled with the generation of free radicals and oxidative injury to tubular cells. The aim of the present study was to assess the effects of erdosteine on prevention of RIN. Th...

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Bibliographic Details
Published in:Environmental toxicology 2011-08, Vol.26 (4), p.395-402
Main Authors: Yesilyurt, Ahmet, Aydın Erden, İsmail, Bilgiç, İsmail, Erden, Gönül, Albayrak, Aynur
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Animals
Antioxidants
Antioxidants - metabolism
Antioxidants - pharmacology
Applied ecology
Biological and medical sciences
blood
Blood Urea Nitrogen
Contrast media
Contrast Media - toxicity
Creatinine
Creatinine - blood
Cystatin C - blood
Ecotoxicology, biological effects of pollution
erdosteine
Female
Free radicals
Fundamental and applied biological sciences. Psychology
General aspects
genes
Injuries
Intravenous administration
intravenous injection
Ischemia
Kidney
Kidney - drug effects
Kidney - metabolism
Kidney - pathology
kidneys
Lcn2
Lipocalin
Lipocalins - genetics
Lipocalins - metabolism
Necrosis
nephrotoxicity
Nitrogen
Osteopontin - genetics
Osteopontin - metabolism
Oxidants
Oxidants - metabolism
Oxidants - toxicity
Oxidative stress
Oxidative Stress - drug effects
Phosphoproteins
Polymerase chain reaction
Prevention
protective effect
radiocontrast nephrotoxicity
Rats
Rats, Wistar
Renal failure
renal toxicity
Reverse Transcriptase Polymerase Chain Reaction
Reverse transcription
RNA
Spp1
Thioglycolates - pharmacology
Thiophenes - pharmacology
Urea
urea nitrogen
Xenobiotics - toxicity
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Summary:Acute renal failure resulting from radiocontrast-induced nephrotoxicity (RIN) is suggested to occur via medullary ischemia coupled with the generation of free radicals and oxidative injury to tubular cells. The aim of the present study was to assess the effects of erdosteine on prevention of RIN. Thirty-three Wistar-albino rats were divided into five groups: control (group 1, n = 6), radiocontrast media (group 2, n = 6), erdosteine (group 3, n = 7), erdosteine four doses before radiocontrast application (group 4, n = 7) and erdosteine one dose at the same day with radiocontrast application (group 5, n = 7). RIN was induced by administration of intravenous high osmolar contrast media amidotrizoate (6 mL/kg). Total RNA was extracted from the kidney, and the expression levels of Lipocalin 2 (Lcn2) and secreted phosphoprotein 1 (Spp1) genes were evaluated by real time reverse transcription polymerase chain reaction (real-time RT-PCR). Total antioxidant status (TAS) and total oxidant status (TOS) were measured in kidney homogenates and serum samples. Serum creatinine, BUN (Blood Urea Nitrogen) and cystatin-C levels were measured from serum samples. The kidneys were evaluated histopathologically. The expression levels of Spp1 and Lcn2 genes in group 2 were significantly higher than groups 1, 3, 4, and 5. The expression levels of Spp1 and Lcn2 genes in group 4 were four and two times lower than group 5, respectively. Kidney TOS levels in group 2 were significantly higher than groups 1, 3, 4, and 5. Kidney TAS levels in group 3 were higher than group 2. Kidney oxidative stress index (OSI) levels in group 2 were significantly higher than groups 4 and 5. All rats in contrast media group developed tubular necrosis, proteinaceous casts, medullary congestion although these changes were significantly reduced in groups 4 and 5. This study demonstrated that multiple doses of erdosteine before application may have higher protective effects against RIN.
ISSN:1520-4081
1522-7278
1522-7278
DOI:10.1002/tox.20691