Inhibitory effects of adlay bran (Coix lachryma-jobi L. var. ma-yuen Stapf) on chemical mediator release and cytokine production in rat basophilic leukemia cells

Adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) has long been used in China to treat rheumatism. We investigated the anti-allergic effects of adlay bran on rat basophilic leukemia (RBL)-2H3 cells. To evaluate the anti-allergic effects of adlay bran, the release of histamines and c...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2012-05, Vol.141 (1), p.119-127
Main Authors: Chen, Hong-Jhang, Lo, Yi-Chen, Chiang, Wenchang
Format: Article
Language:English
Subjects:
Acetates - chemistry
Adlay bran
AKT protein
Allergic response
Animals
Anti-Allergic Agents - chemistry
Anti-Allergic Agents - isolation & purification
Anti-Allergic Agents - pharmacology
Basophilic leukemia
beta-N-Acetylhexosaminidases - metabolism
Blotting, Western
Cell Line, Tumor
Cell Survival - drug effects
Coix
Coix - chemistry
Cytokines
Cytokines - metabolism
Degranulation
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Ethanol - chemistry
Ethyl acetate
Flavones
Histamine
Histamine Release - drug effects
Hypersensitivity
Inflammation Mediators - metabolism
Interleukin 6
Interleukin-4 - metabolism
Interleukin-6 - metabolism
Leukemia, Basophilic, Acute - immunology
Luteolin - isolation & purification
Luteolin - pharmacology
Mast cells
Oxidative Stress - drug effects
phenolic acids
Phenols - isolation & purification
Phenols - pharmacology
Phosphorylation
Phytotherapy
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plants, Medicinal
Protein kinase C
Protein Kinase C - metabolism
Proto-Oncogene Proteins c-akt - metabolism
Rats
Reactive oxygen species
Reactive Oxygen Species - metabolism
Rheumatism
Seeds
Signal transduction
Signal Transduction - drug effects
Solvents - chemistry
Tears
Tumor necrosis factor- alpha
Tumor Necrosis Factor-alpha - metabolism
Western blotting
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Summary:Adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) has long been used in China to treat rheumatism. We investigated the anti-allergic effects of adlay bran on rat basophilic leukemia (RBL)-2H3 cells. To evaluate the anti-allergic effects of adlay bran, the release of histamines and cytokines were measured using ELISA. To explore the mechanism of these effects, the protein expression levels were determined using western blotting. A 40.8μg/mL concentration of the ethyl acetate fraction of the ethanolic extracts of adlay bran (ABE-EtOAc) effectively inhibited mast cell degranulation. The 40–100% EtOAc/Hex subfractions of ABE-EtOAc inhibited histamine release with an IC50 of 71–87μg/mL. Moreover, the ABE-EtOAc subfractions suppressed the secretion of interleukin (IL)-4, IL-6 and tumor necrosis factor-α in the RBL-2H3 cells, indicating that adlay bran can inhibit cytokine secretion in the late phase of the allergic reaction. In addition, adlay bran reduced the intracellular production of reactive oxygen species, inhibited the phosphorylation of Akt and decreased the expression of protein kinase C. Furthermore, six phenolic acids and one flavone were isolated. Of these compounds, luteolin showed the most potent inhibitory activity (IC50=1.5μg/mL). Adlay bran extract reduced the release of histamines and cytokines and suppressed the production of Akt. These combined effects influenced the signal transduction in RBL-2H3 cells, thereby revealing the mechanisms of the anti-allergic effects of adlay.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2012.02.009