Licochalcone A Prevents Adipocyte Differentiation and Lipogenesis via Suppression of Peroxisome Proliferator-Activated Receptor γ and Sterol Regulatory Element-Binding Protein Pathways

Licochalcone A (LA) has been shown to exert multiple pharmacological effects, including anti-inflammatory, antiparasitic, antifungal, anticancer, and osteogenic activities. The present study investigated the ability of LA to suppress the differentiation of 3T3-L1 preadipocytes, and its antiobesity a...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2012-05, Vol.60 (20), p.5112-5120
Main Authors: Quan, Hai-Yan, Baek, Nam In, Chung, Sung Hyun
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipocytes - chemistry
Adipocytes - cytology
Adipocytes - metabolism
Animals
Anti-Obesity Agents - pharmacology
Biological and medical sciences
biomarkers
body weight
Cell Differentiation - drug effects
Chalcones - pharmacology
clinical trials
Diet, High-Fat
fatty acid-binding proteins
fatty acids
fatty-acid synthase
Food industries
Fundamental and applied biological sciences. Psychology
gene expression regulation
glycerol-3-phosphate acyltransferase
high fat diet
Intra-Abdominal Fat - drug effects
Lipids - analysis
Lipids - biosynthesis
lipogenesis
Lipogenesis - drug effects
Mice
Mice, Inbred ICR
micro-computed tomography
PPAR gamma - antagonists & inhibitors
stearoyl-CoA desaturase
Sterol Regulatory Element Binding Proteins - antagonists & inhibitors
visceral fat
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Summary:Licochalcone A (LA) has been shown to exert multiple pharmacological effects, including anti-inflammatory, antiparasitic, antifungal, anticancer, and osteogenic activities. The present study investigated the ability of LA to suppress the differentiation of 3T3-L1 preadipocytes, and its antiobesity activity was explored using high fat diet (HFD)-fed ICR mice. During the terminal differentiation process, 3T3-L1 preadipocytes were treated with LA, and the lipid contents were quantified along with any changes in the expression of biomarkers associated with adipocyte differentiation and lipogenesis. The results show that LA significantly reduced lipid accumulation and down-regulated the expression of peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, sterol regulatory element-binding protein 1c, and their target genes (fatty acid binding protein, fatty acid synthase, stearoyl-CoA desaturase 1, and glycerol-3-phosphate acyltransferase). In an animal study, body weight, triglyceride, cholesterol, and nonesterified fatty acid levels in the group given 10 mg/kg LA were significantly decreased by 14.0, 48.2, 58.9, and 73.5%, respectively. Transverse microcomputed tomography indicated that visceral fat depots in LA-treated mice were markedly reduced when compared with those of the HFD control group. In summary, these results suggest that LA exerts an antiobesity effect and that it is a candidate for future clinical trials.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf2050763