BRG1 expression is increased in human glioma and controls glioma cell proliferation, migration and invasion in vitro

Purpose The purposes of our study were to elucidate the role of BRG1 in the development of human glioma and to determine the effect of BRG1 on glioma cell growth, migration and invasion. Methods Using tissue microarray and immunohistochemistry, we evaluated BRG1 staining in 190 glioma tissues, 8 nor...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2012-06, Vol.138 (6), p.991-998
Main Authors: Bai, Jin, Mei, Peng-Jin, Liu, Hui, Li, Chen, Li, Wang, Wu, Yong-Ping, Yu, Zheng-Quan, Zheng, Jun-Nian
Format: Article
Language:English
Subjects:
Antineoplastic agents
Biological and medical sciences
Brain
Brain Neoplasms - genetics
Brain Neoplasms - metabolism
Brain Neoplasms - pathology
Cancer Research
Cell Cycle - genetics
Cell Growth Processes - physiology
Cell Line, Tumor
Cell Movement - physiology
Cellular biology
Cyclin B1 - genetics
Cyclin B1 - metabolism
Cyclin D1 - genetics
Cyclin D1 - metabolism
DNA Helicases - biosynthesis
DNA Helicases - genetics
DNA Helicases - metabolism
Female
G1 Phase Cell Cycle Checkpoints - genetics
Gene expression
Gene Knockdown Techniques - methods
Gene Silencing
Glioma - genetics
Glioma - metabolism
Glioma - pathology
Hematology
Humans
Immunohistochemistry - methods
Internal Medicine
Male
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 2 - metabolism
Medical sciences
Medicine
Medicine & Public Health
Middle Aged
Neoplasm Invasiveness
Neurology
Nuclear Proteins - biosynthesis
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Oncology
Original Paper
Pharmacology. Drug treatments
RNA, Small Interfering - genetics
Sincalide - genetics
Sincalide - metabolism
Transcription Factors - biosynthesis
Transcription Factors - genetics
Transcription Factors - metabolism
Tumors
Tumors of the nervous system. Phacomatoses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose The purposes of our study were to elucidate the role of BRG1 in the development of human glioma and to determine the effect of BRG1 on glioma cell growth, migration and invasion. Methods Using tissue microarray and immunohistochemistry, we evaluated BRG1 staining in 190 glioma tissues, 8 normal brain tissues and 8 tumor adjacent normal brain tissues. We studied glioma cell proliferative ability with reduced BRG1 expression by siRNA using CCK-8 cell proliferation assay and cell cycle analysis. We studied the role of BRG1 in glioma cell migration and invasion by cell migration assay and matrigel invasion assay. We performed western blot to detect cyclin D1, cyclin B1 and MMP-2 protein expression. We also detected MMP-2 enzyme activity by gelatin zymography. Results Our results showed that BRG1 expression was increased in benign tumor and malignant tumor compared with tumor adjacent normal brain tissue ( P  
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-012-1172-8