Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo

Problem Bacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage‐associated molecular patterns. So, th...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2012-06, Vol.67 (6), p.526-539
Main Authors: Borges, Álan Maia, Healey, Gareth David, Sheldon, Iain Martin
Format: Article
Language:English
Subjects:
Animals
Arcanobacterium
Bacterial Infections - immunology
Bacterial Infections - microbiology
Bacterial Infections - veterinary
Bovine
Cattle
Cattle Diseases - immunology
Cattle Diseases - microbiology
Corynebacterium
Cytokines - immunology
endometrium
Endometrium - immunology
Escherichia coli
explant
Female
Immunity, Innate
inflammation
Inflammation - immunology
innate immunity
Uterine Diseases - immunology
Uterine Diseases - microbiology
Uterine Diseases - veterinary
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Summary:Problem Bacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage‐associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation. Method of study Intact bovine endometrium explants were collected using a sterile 8‐mm punch biopsy and cultured ex vivo with bacteria or pathogen‐associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, TdT‐mediated biotin–dUTP nick‐end labelling and lactate dehydrogenase assay. Results Intact endometrium explants accumulated IL‐6, IL‐1β and IL‐8 in response to Gram‐negative or Gram‐positive bacteria, and their purified pathogen‐associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium. Conclusion This study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.
ISSN:1046-7408
1600-0897
DOI:10.1111/j.1600-0897.2012.01106.x