Zoledronic acid inhibits proliferation and impairs migration and invasion through downregulating VEGF and MMPs expression in human nasopharyngeal carcinoma cells

To demonstrate the effect of zoledronic acid in proliferation, invasion, and migration of human nasopharyngeal carcinoma cell HNE-1 and explore the potential role of VEGF, MMP-2, and MMP-9 proteins in vitro. Human nasopharyngeal carcinoma cell HNE-1 was exposed to various concentrations (0–40 μmol/l...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2012-06, Vol.29 (2), p.714-720
Main Authors: Li, Xu-yuan, Lin, Ying-cheng, Huang, Wan-lan, Hong, Chao-qun, Chen, Jiong-yu, You, Yan-jie, Li, Wei-bing
Format: Article
Language:English
Subjects:
Blotting, Western
Bone Density Conservation Agents - pharmacology
Carcinoma
Cell Adhesion - drug effects
Cell Movement - drug effects
Cell Proliferation - drug effects
Diphosphonates - pharmacology
Down-Regulation
Enzyme-Linked Immunosorbent Assay
Hematology
Humans
Imidazoles - pharmacology
Internal Medicine
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - genetics
Matrix Metalloproteinase 9 - metabolism
Medicine
Medicine & Public Health
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms - genetics
Nasopharyngeal Neoplasms - metabolism
Nasopharyngeal Neoplasms - pathology
Neoplasm Invasiveness
Oncology
Original Paper
Pathology
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A - genetics
Vascular Endothelial Growth Factor A - metabolism
Zoledronic Acid
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Summary:To demonstrate the effect of zoledronic acid in proliferation, invasion, and migration of human nasopharyngeal carcinoma cell HNE-1 and explore the potential role of VEGF, MMP-2, and MMP-9 proteins in vitro. Human nasopharyngeal carcinoma cell HNE-1 was exposed to various concentrations (0–40 μmol/l) of zoledronic acid. Zoledronic acid inhibited proliferation of HNE-1 cells though not in a dose-dependent manner. Zoledronic acid had exerted a dose-dependent effect on the migration and invasion of HNE-1 cells. Both expressions of mRNA and protein of MMP2, MMP9, and VEGF were reduced, respectively, detected by RT-PCR and Western blot assays. These data suggested that zoledronic acid not only inhibited growth but also invasion and migration of HNE-1 cells in vitro. The anti-cancer action of zoledronic acid was partially associated with the suppression of VEGF expression and secretion and downregulating the expression of MMP2 and MMP9.
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-011-9904-1