State-of-the Art Comparability of Corrected Emission Spectra. 2. Field Laboratory Assessment of Calibration Performance Using Spectral Fluorescence Standards

In the second part of this two-part series on the state-of-the-art comparability of corrected emission spectra, we have extended this assessment to the broader community of fluorescence spectroscopists by involving 12 field laboratories that were randomly selected on the basis of their fluorescence...

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Published in:Analytical chemistry (Washington) 2012-05, Vol.84 (9), p.3899-3907
Main Authors: Resch-Genger, Ute, Bremser, Wolfram, Pfeifer, Dietmar, Spieles, Monika, Hoffmann, Angelika, DeRose, Paul C, Zwinkels, Joanne C, Gauthier, François, Ebert, Bernd, Taubert, R. Dieter, Voigt, Jan, Hollandt, Jörg, Macdonald, Rainer
Format: Article
Language:English
Subjects:
Accuracy
Analytical chemistry
Calibration
Chemistry
Exact sciences and technology
Fluorescence
General, instrumentation
Spectrometric and optical methods
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Summary:In the second part of this two-part series on the state-of-the-art comparability of corrected emission spectra, we have extended this assessment to the broader community of fluorescence spectroscopists by involving 12 field laboratories that were randomly selected on the basis of their fluorescence measuring equipment. These laboratories performed a reference material (RM)-based fluorometer calibration with commercially available spectral fluorescence standards following a standard operating procedure that involved routine measurement conditions and the data evaluation software LINKCORR developed and provided by the Federal Institute for Materials Research and Testing (BAM). This instrument-specific emission correction curve was subsequently used for the determination of the corrected emission spectra of three test dyes, X, QS, and Y, revealing an average accuracy of 6.8% for the corrected emission spectra. This compares well with the relative standard uncertainties of 4.2% for physical standard-based spectral corrections demonstrated in the first part of this study (previous paper in this issue) involving an international group of four expert laboratories. The excellent comparability of the measurements of the field laboratories also demonstrates the effectiveness of RM-based correction procedures.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac203451g