Homo-catiomer integration into PEGylated polyplex micelle from block-catiomer for systemic anti-angiogenic gene therapy for fibrotic pancreatic tumors

Abstract Homo-poly{ N ′-[ N -(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H ] was attempted to integrate into poly (ethylene glycol) (PEG)- b -PAsp(DET)] ( B ) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration....

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2012-06, Vol.33 (18), p.4722-4730
Main Authors: Chen, Qixian, Osada, Kensuke, Ishii, Takehiko, Oba, Makoto, Uchida, Satoshi, Tockary, Theofilus A, Endo, Taisuke, Ge, Zhishen, Kinoh, Hiroaki, Kano, Mitsunobu R, Itaka, Keiji, Kataoka, Kazunori
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cell Line
Cell Survival - drug effects
Dentistry
DNA
Flow Cytometry
Gene transfer
Genetic Therapy - methods
Humans
In vitro test
In vivo test
Mice
Mice, Nude
Micelle
Micelles
Microscopy, Electron, Transmission
Nanoparticle
Nanoparticles - adverse effects
Nanoparticles - chemistry
Pancreatic Neoplasms - therapy
Polyethylene Glycols - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Homo-poly{ N ′-[ N -(2-aminoethyl)-2-aminoehtyl]aspartamide} [PAsp(DET), H ] was attempted to integrate into poly (ethylene glycol) (PEG)- b -PAsp(DET)] ( B ) formulated polyplex micelle with the aim of enhancing cell transfection efficiency for PEGylated polyplex micelle via H integration. In vitro evaluations verified H integration of potent stimulation in enhancing cell-transfecting activity of PEGylated polyplex micelles via promoted cellular uptake and facilitated endosome escape. In vivo anti-angiogenic tumor suppression evaluations validated the feasibility of H integration in promoting gene transfection to the affected cells via systemic administration, where loaded anti-angiogenic gene remarkably expressed in the tumor site, thereby imparting significant inhibitory effect on the growth of vascular endothelial cells, ultimately leading to potent tumor growth suppression. These results demonstrated potency of H integration for enhanced transfection activity and potential usage in systemic applications, which could have important implications on the strategic use of H integration in the non-viral gene carrier design.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.03.017