The effects of inhibiting leukocyte migration with fucoidin in a rat peritonitis model

To study the effects of fucoidin on leukocyte rolling and emigration and bacterial colonization in a peritonitis sepsis model in rats. A controlled study in 64 male Wistar rats, anesthetized and rendered septic by cecal ligation and puncture (CLP). Immediately after CLP 32 animals received a continu...

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Bibliographic Details
Published in:Intensive care medicine 2000-10, Vol.26 (10), p.1540-1546
Main Authors: LINNEMANN, G, REINHART, K, PARADE, U, PHILIPP, A, PFISTER, W, STRAUBE, E, KARZAI, W
Format: Article
Language:English
Subjects:
Abdomen
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Bacterial Infections - drug therapy
Bacterial Infections - immunology
Bacterial Infections - microbiology
Bacterial Infections - mortality
Biological and medical sciences
Chemotaxis, Leukocyte - drug effects
Colony Count, Microbial
Disease Models, Animal
Drug Evaluation, Preclinical
Emergency and intensive care: infection, septic shock
Emigration
Infections
Inflammation
Infusions, Intravenous
Intensive care
Intensive care medicine
Isotonic Solutions - pharmacology
Isotonic Solutions - therapeutic use
Leukocyte Count
Leukocytes
Leukocytosis - blood
Leukocytosis - chemically induced
Liver
Lungs
Male
Medical sciences
Microscopy
Neutrophil Activation - drug effects
Neutrophils
Nitrous oxide
Peritonitis
Peritonitis - drug therapy
Peritonitis - immunology
Peritonitis - microbiology
Peritonitis - mortality
Polysaccharides - pharmacology
Polysaccharides - therapeutic use
Rats
Rats, Wistar
Ringer's Lactate
Selectins - drug effects
Sepsis
Sepsis - drug therapy
Sepsis - immunology
Sepsis - microbiology
Sepsis - mortality
Survival Analysis
Time Factors
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Summary:To study the effects of fucoidin on leukocyte rolling and emigration and bacterial colonization in a peritonitis sepsis model in rats. A controlled study in 64 male Wistar rats, anesthetized and rendered septic by cecal ligation and puncture (CLP). Immediately after CLP 32 animals received a continuous infusion of fucoidin and 32 a continuous infusion of Ringer's lactate. Systemic leukocyte counts were determined every 2 h after CLP. Surviving animals were anesthetized 24 h after CLP, and intravital measurements of leukocyte rolling in venules in the cremaster muscle were performed. The animals were then killed and their organs harvested for histological and microbiological examinations. The 24-h survival was comparable in the two groups. Fucoidin-treated animals had higher leukocyte counts in the systemic circulation and lower counts in the lungs, liver, abdominal cavity, and brain than control animals. The number of bacterial colony forming units in the abdominal cavity, lungs, liver, brain and blood did not differ in the two groups. Fucoidin treatment changed the type of bacteria predominantly found in the examined organs from Escherichia coli to Pseudomonas aeruginosa. In an intra-abdominal model of sepsis we found that treatment with fucoidin induces leukocytosis inhibits leukocyte rolling and reduces leukocyte emigration in the abdominal cavity, lungs, and liver. Reduction in the number of emigrating leukocytes was not associated with an increase in bacterial counts found in the examined organs.
ISSN:0342-4642
1432-1238
DOI:10.1007/s001340000642