RKIP inhibits the migration and invasion of human prostate cancer PC-3M cells through regulation of extracellular matrix

Raf kinase inhibitor protein (RKIP) plays a pivotal role in several intracellular signaling cascades and has been implicated as a metastasis suppressor in multiple cancer cells including prostate cancer cells, but the mechanism is not very clear. In this study, we investigated the effect of RKIP on...

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Bibliographic Details
Published in:Molecular biology (New York) 2011-12, Vol.45 (6), p.921-928
Main Authors: Xinzhou, H., Ning, Y., Ou, W., Xiaodan, L., Fumin, Y., Huitu, L., Wei, Z.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell adhesion & migration
Gene expression
Human Genetics
Life Sciences
Molecular biology
Molecular Biology of the Cell
Prostate cancer
Proteins
Signal transduction
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Summary:Raf kinase inhibitor protein (RKIP) plays a pivotal role in several intracellular signaling cascades and has been implicated as a metastasis suppressor in multiple cancer cells including prostate cancer cells, but the mechanism is not very clear. In this study, we investigated the effect of RKIP on cell proliferation, migration and invasion using human prostate cancer PC-3M cells as a model system. Our results indicate that RKIP does not effect cell proliferation in PC-3M cells, but inhibits both cell migration and cell invasion. In association with this inhibitory effect, RKIP down-regulates matrix metalloproteinases (MMP-2 and MMP-9), cathepsin B and urinary plasminogen activator (uPA). Also RKIP has the ability to regulate the expression of E-cadherin. But ectopic expression of RKIP does not affect the level of the Snail protein. As it has been indicated here, RKIP inhibits the migration and invasion ability of human prostate cancer cells through regulation of the extracellular matrix. These findings provide new mechanistic insight how RKIP suppresses metastasis in vitro.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893311060197