Complete Biodegradation of 4-Fluorocinnamic Acid by a Consortium Comprising Arthrobacter sp. Strain G1 and Ralstonia sp. Strain H1

A consortium of the newly isolated bacterial strains Arthrobacter sp. strain G1 and Ralstonia sp. strain H1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. Strain G1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2011-01, Vol.77 (2), p.572-579
Main Authors: Hasan, Syed A, Ferreira, Maria Isabel M, Koetsier, Martijn J, Arif, Muhammad I, Janssen, Dick B
Format: Article
Language:English
Subjects:
Acids
Aerobic conditions
Anaerobiosis
Arthrobacter
Arthrobacter - classification
Arthrobacter - genetics
Arthrobacter - isolation & purification
Arthrobacter - metabolism
Benzoates - metabolism
Biodegradation
Biological and medical sciences
Biotransformation
Cinnamates - metabolism
Cluster Analysis
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Enzymes
Fluorides - metabolism
Fundamental and applied biological sciences. Psychology
Gram-positive bacteria
Metabolic Networks and Pathways - genetics
Microbiology
Molecular Sequence Data
Oxidation
Phylogeny
Proteins
Ralstonia - classification
Ralstonia - genetics
Ralstonia - isolation & purification
Ralstonia - metabolism
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Signal transduction
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Summary:A consortium of the newly isolated bacterial strains Arthrobacter sp. strain G1 and Ralstonia sp. strain H1 utilized 4-fluorocinnamic acid for growth under aerobic conditions. Strain G1 converted 4-fluorocinnamic acid into 4-fluorobenzoic acid and used the two-carbon side chain for growth, with some formation of 4-fluoroacetophenone as a dead-end side product. In the presence of strain H1, complete mineralization of 4-fluorocinnamic acid and release of fluoride were obtained. Degradation of 4-fluorocinnamic acid by strain G1 occurred through a β-oxidation mechanism and started with the formation of 4-fluorocinnamoyl-coenzyme A (CoA), as indicated by the presence of 4-fluorocinnamoyl-CoA ligase. Enzymes for further transformation were detected in cell extract, i.e., 4-fluorocinnamoyl-CoA hydratase, 4-fluorophenyl-β-hydroxy propionyl-CoA dehydrogenase, and 4-fluorophenyl-β-keto propionyl-CoA thiolase. Degradation of 4-fluorobenzoic acid by strain H1 proceeded via 4-fluorocatechol, which was converted by an ortho-cleavage pathway.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.00393-10