Novel Photodynamic Therapy Using Water-dispersed TiO^sub 2^-Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro

Titanium dioxide (TiO^sub 2^) is thought to be a photocatalytic agent excited by UV light. Our aim was to investigate the photocatalytic antitumor effect of water-dispersed TiO^sub 2^ nanoparticles on C6 rat glioma cells and to evaluate the treatment responses by the spheroid models. Water-dispersed...

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Bibliographic Details
Published in:Photochemistry and photobiology 2010-07, Vol.86 (4), p.964
Main Authors: Yamaguchi, Shigeru, Kobayashi, Hiroyuki, Narita, Takuhito, Kanehira, Koki, Sonezaki, Shuji, Kubota, Yoshinobu, Terasaka, Shunsuke, Iwasaki, Yoshinobu
Format: Article
Language:English
Subjects:
Apoptosis
Atoms & subatomic particles
Dopamine
Molecular weight
Photodynamic therapy
Studies
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Summary:Titanium dioxide (TiO^sub 2^) is thought to be a photocatalytic agent excited by UV light. Our aim was to investigate the photocatalytic antitumor effect of water-dispersed TiO^sub 2^ nanoparticles on C6 rat glioma cells and to evaluate the treatment responses by the spheroid models. Water-dispersed TiO^sub 2^ nanoparticles were constructed by the adsorption of chemical modified polyethylene glycol (PEG) on the TiO^sub 2^ surface (TiO^sub 2^/PEG). Each monolayer and spheroid of C6 cells was coincubated with various concentrations of TiO^sub 2^/PEG and subsequently irradiated with UV light. Damage of the cells and spheroids was evaluated sequentially by staining with the fluorescent dyes. The cytotoxic effect was correlated with the concentration of TiO^sub 2^/PEG and the energy dose of UV irradiation. More than 90% of cells were killed after 13.5 J cm^sup -2^ of UV irradiation in the presence of 500 µg mL^sup -1^ TiO^sub 2^/PEG. The irradiated spheroids in the presence of TiO^sub 2^/PEG showed growth suppression compared with control groups. In TiO^sub 2^/PEG-treated spheroids, the number of Annexin V-FITC-stained cells gradually increased during the first 6 h, and subsequently propidium iodide-stained cells appeared. The results of this study suggest that newly developed photoexcited TiO^sub 2^/PEG have antitumoral activity. Photodynamic therapy utilizing this material can he a clue to a novel therapeutic strategy for glioma. [PUBLICATION ABSTRACT]
ISSN:0031-8655
1751-1097