contribution to the classification of evergreen azalea cultivars located in the Lake Maggiore area (Italy) by means of AFLP markers

The amplified fragment length polymorphism (AFLP) technique was employed to assess the genetic conformity within a gene pool of evergreen azaleas. Ninety-three genotypes grown in historical gardens and nurseries of the Lake Maggiore area (Northern Italy), locally classified in the groups Indica, Jap...

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Bibliographic Details
Published in:Euphytica 2007-11, Vol.158 (1-2), p.47-66
Main Authors: Scariot, Valentina, Handa, Takashi, De Riek, Jan
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
amplified fragment length polymorphism
Biological and medical sciences
Cultivars
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
genetic distance
genetic markers
genetic variation
Genetics
Genetics and breeding of economic plants
genotype
molecular systematics
nursery crops
Origin, evolution, domestication
ornamental woody plants
pedigree
phylogeny
plant genetic resources
Plant material
plant morphology
plant taxonomy
population genetics
Rhododendron
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Summary:The amplified fragment length polymorphism (AFLP) technique was employed to assess the genetic conformity within a gene pool of evergreen azaleas. Ninety-three genotypes grown in historical gardens and nurseries of the Lake Maggiore area (Northern Italy), locally classified in the groups Indica, Japonica and Amoena, 44 reference cultivars of the four more or less universally recognized groups Belgian, Hirado, Kurume and Satsuki, and 40 species and species accessions that most concurred in the origin of the reference groups were included. Aims of the study were to define the genetic profiles of the Italian accessions and to provide useful information for their classification and pedigree reconstruction. Ordination analyses were used to evaluate if the AFLP technique can be applied to establish phylogenetic relationships and to investigate the DNA-based relatedness within the Italian gene pool and between the latter and the reference one. The results regarding the Italian accessions were compared with morphological data by means of Mantel's test. Assignment tests both on the level of the groups and on individual plant level for the reference and Italian genotypes were applied to further evaluate the relatedness of the groups or individual accessions. Results demonstrated that the AFLP technique together with morphological characterization can contribute to depict the phylogeny of the evergreen azalea. Comparing the reference and Italian genotypes, conclusions about the classification of the evergreen azalea cultivars located in the Lake Maggiore area were drawn.
ISSN:0014-2336
1573-5060
DOI:10.1007/s10681-007-9425-3