Arsenic-contaminated soils genetically modifiedPseudomonas spp. and their arsemc-phytoremediation potential

Sorghum was inoculated withPseudomonas bacteria, including strains harboring an As-resistance plasmid, pBS3031, to enhance As-extraction by the plants.Pseudomonas strains (P.fluorescens 38a, P.putida 53a, and P.aureofaciens BS1393) were chosen because they are antagonistic to a wide range of phyto-p...

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Published in:Journal of soils and sediments 2002-03, Vol.2 (1), p.19-23
Main Authors: Sizova, Olga I., Kochetkov, Vladimir V., Validov, Shamil Z., Boronin, Alexander M., Kosterin, Paul V., Lyubun, Yelena V.
Format: Article
Language:English
Subjects:
Arsenate reductase
Arsenates
Arsenic
Arsenite
Bacteria
Bioremediation
Conjugation
Fungi
Genetic modification
Genetic transformation
Pathogens
Phytoremediation
Plant extracts
Plant growth
Plant roots
Plants
Plasmids
Reductases
Resistance mechanisms
Rhizosphere
Sediment pollution
Sodium
Sodium arsenite
Soil
Soil contamination
Soil pollution
Soils
Sorghum
Strains
Strains (organisms)
Survivability
Survival
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Summary:Sorghum was inoculated withPseudomonas bacteria, including strains harboring an As-resistance plasmid, pBS3031, to enhance As-extraction by the plants.Pseudomonas strains (P.fluorescens 38a, P.putida 53a, and P.aureofaciens BS1393) were chosen because they are antagonistic to a wide range of phyto-pathogenic fungi and bacteria, and they can stimulate plant growth. The resistance of natural rhizospheric pseudomonads to sodium arsenite was assessed. Genetically modifiedPseudomonas strains resistant to As(III)/As(V) were obtained via conjugation or transformation. The effects of the strains on the growth of sorghum on sodium-arsenite-containing soils were assessed. The conclusions from this study are: (1) It is possible to increase the survivability of sorghum growing in sodium-arsenite-containing soil by using rhizosphere pseudomonads. (2) The presence of pBS3031 offers the strains a certain selective advantage in arsenite-contaminated soil. (3) The presence of pBS3031 impairs plant growth, due to the As-resistance mechanism determined by this plasmid: the transformation of the less toxic arsenate into the more toxic, plant-root-available arsenite by arsenate reductase and the active removal of arsenite from bacterial cells. (4) Such a mechanism makes it possible to develop a bacteria-assisted phytoremediation technology for the cleanup of As-contaminated soils and is the only possible way of removing the soil-sorbed arsenates from the environment.
ISSN:1439-0108
1614-7480
DOI:10.1007/BF02991246