Somatic embryogenesis and plant regeneration in two wild cotton species belong to G genome

The present work describes the plant regeneration via somatic embryogenesis in two wild cotton species belonging to G genome: Gossypium nelsonii Fryx and Gossypium australe F Muell. The role of plant hormones and carbohydrates was also evaluated for somatic embryogenesis and somatic embryo developme...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2010-06, Vol.46 (3), p.298-305
Main Authors: Yan, Shu-Feng, Zhang, Qiao, Wang, Jing-Er, Sun, Yu-Qiang, Daud, M. K, Zhu, Shui-Jin
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Callus
Cell Biology
Cotton
DEVELOPMENTAL BIOLOGY
Embryogenesis
Embryos
Genetics
Genomes
Highlands
Life Sciences
Plant Breeding/Biotechnology
Plant cells
Plant Genetics and Genomics
Plant Sciences
Plant Tissue Culture
Plants
Regeneration
Seeds
Somatic embryogenesis
Somatic embryos
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Summary:The present work describes the plant regeneration via somatic embryogenesis in two wild cotton species belonging to G genome: Gossypium nelsonii Fryx and Gossypium australe F Muell. The role of plant hormones and carbohydrates was also evaluated for somatic embryogenesis and somatic embryo development. Normal plants were obtained from G. nelsonii Fryx; abnormal plants and somatic embryos were obtained from G. australe F Muell. The best medium for callus induction for these G genome wild cotton species was MSB₅ supplemented with 0.1 mg L⁻¹ KT and 0.1 mg L⁻¹ 2,4-D. For embryogenic callus proliferation, the best medium used was MSB₅ supplemented with 0.2 mg L⁻¹ KT and 0.5 mg L⁻¹ IBA. The medium MSB₅ supplemented with 0.15 mg L⁻¹ KT and 0.5 mg L⁻¹ NAA was used successfully for root initiation and plant growth. In addition, adding CuSO₄ and AgNO₃ in the callus-inducing and proliferation medium resulted in a number of somatic embryos. Glucose and maltose, the carbon sources in somatic culture, were used for callus induction, but maltose worked even better than glucose for proliferation of embryogenic callus and development of somatic embryos.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-009-9270-2