Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway

Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell—specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helica...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2010-06, Vol.107 (26), p.11841-11846
Main Authors: Zheng, Ke, Xiol, Jordi, Reuter, Michael, Eckardt, Sigrid, Leu, N. Adrian, McLaughlin, K. John, Stark, Alexander, Sachidanandam, Ravi, Pillai, Ramesh S., Wang, Peijing Jeremy, Hogan, Brigid L. M.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Argonaute Proteins
Base Sequence
Biological Sciences
Biosynthesis
Cells
Derepression
DNA Methylation
DNA Primers - genetics
Drosophila
Fertility
Genetics
Germ cells
Male
Meiosis
Mice
Mice, Knockout
Mutation
Pachytene stage
Proteins
Proteins - metabolism
Retroelements - genetics
Retrotransposons
Ribonucleoproteins, Small Nuclear - genetics
Ribonucleoproteins, Small Nuclear - metabolism
RNA
RNA helicase
RNA Helicases - deficiency
RNA Helicases - genetics
RNA Helicases - metabolism
RNA, Small Interfering - genetics
RNA, Small Interfering - metabolism
Rodents
Spermatocytes
Spermatocytes - metabolism
Spermatogenesis
Spermatogonia
Spermatogonia - metabolism
Testes
Testis - metabolism
Transposons
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Summary:Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell—specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicase domain in mice renders both MILI and MIWI2 devoid of piRNAs. Absence of a functional piRNA pathway in Mov10l1 mutant testes causes loss of DNA methylation and subsequent derepression of retrotransposons in germ cells. The Mov10l1 mutant males are sterile owing to complete meiotic arrest. This mouse mutant expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1003953107