Pathogenicity, resistance patterns, virulence traits, and resistance genes of re-emerging extensively drug-resistant (XDR) Aeromonas veronii in Oreochromis niloticus

Aeromonas veronii , a threatening fish pathogen, is implicated in food-borne infections in humans, contributing to substantial economic losses in the aquaculture sector. To examine the occurrence, resistance profiles, 16SrRNA sequence analysis, virulence-determinant and resistance genes, and pathoge...

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Published in:Aquaculture international 2024-10, Vol.32 (5), p.6987-7006
Main Authors: Ibrahim, Ghada A., Mabrok, Mahmoud, Alfifi, Khyreyah J., Alatawy, Marfat, Al-otaibi, Amenah S., Alenzi, Asma Massad, Abdel Rahman, Afaf N., El-Malt, Rania M.S., Ibrahim, Sarah A., El-Tarabili, Reham M., Algammal, Abdelazeem M.
Format: Article
Language:English
Subjects:
Aeromonas veronii
Aquaculture
Biomedical and Life Sciences
Commercial farms
Drug resistance
Drugs
Fish
Foodborne diseases
Freshwater & Marine Ecology
Genes
Health hazards
Life Sciences
Marine fishes
Nucleotide sequence
Oreochromis niloticus
Pathogens
PCR
Phylogenetics
Public health
Virulence
Zoology
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Summary:Aeromonas veronii , a threatening fish pathogen, is implicated in food-borne infections in humans, contributing to substantial economic losses in the aquaculture sector. To examine the occurrence, resistance profiles, 16SrRNA sequence analysis, virulence-determinant and resistance genes, and pathogenicity of A. veronii isolated from Nile tilapia ( Oreochromis niloticus ), almost 150 fish weighing 75 ± 10 g (apparently healthy; n = 50 and moribund fish; n = 100) were collected from commercial farms in Ismailia Governorate, Egypt. Subsequently, the clinical, post-mortem, and bacteriological examinations were carried out. The overall prevalence of A. veronii in the collected O. niloticus was 18.6% (28/150), where the pathogen was only detected in the moribund one. Phylogenetic analysis revealed that the tested isolate exhibited significant genetic identity with other strains from diverse locations and origins in China and Egypt. PCR showed that aer A (95.4%) was the most predominant virulence gene associated with A. veronii isolates retrieved from O. niloticus , followed by hly (80.3%), ast and ser (69.7% for each), and alt (50.1%). Moreover, 40.9% of the obtained isolates were extensively drug-resistant (XDR) to seven classes and inherited bla TEM , tet A, bla CTX-M , and sul 1 genes. Besides, 6.1% of the recovered isolates were carbapenem-resistant and XDR to seven classes harboring bla TEM , bla NDM , bla CTX-M , sul 1, and tet A genes. The cumulative mortality rate was 86.7% in the fish group injected with a virulent A. veronii strain at a concentration of 3 × 10 8 CFU/mL. Concisely, this study underscored the existence of XDR A. veronii in O. niloticus specifying a public health hazard. XDR A. veronii isolated from O. niloticus commonly inherited aer A, ast , ser , and alt virulence-determinant genes and bla TEM , sul 1, bla CTX-M , and tet A resistance genes.
ISSN:0967-6120
1573-143X
DOI:10.1007/s10499-024-01498-0