Biosynthesis of actinobacterial mediated silver nanoparticle (AgNPs): therapeutic potential and in-silico docking analysis on targeted virulence receptor

The study of nanoparticles has gained popularity recently, particularly in biomedicine where metallic AgNPs were widely utilized for their therapeutic benefits. This current study focuses on biosynthesis of AgNPs using marine Streptomyces coelicoflavus MTK30, detailing their characterization, in-vit...

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Published in:Journal of sol-gel science and technology 2024-07, Vol.111 (1), p.293-308
Main Authors: Elumalai, Lokesh, Anbazhagan, Ganesh Kumar, Palaniyandi, Sankarganesh, Nagarajan, Siddharthan, Murthy, Sangeetha, Anbalmani, Sivarajan, Mohanam, Nithyalakshmi, Munusamy, Ayyasamy Pudukadu, Ramasamy, Balagurunathan
Format: Article
Language:English
Subjects:
Albinism
Antiinfectives and antibacterials
Bacteria
Biosynthesis
Cancer
Ceramics
Chemistry and Materials Science
Composites
Glass
Inorganic Chemistry
Materials Science
Molecular docking
Nanoparticles
Nanotechnology
Natural Materials
Optical and Electronic Materials
Original Paper
Proteins
Scavenging
Silver
Surface plasmon resonance
Virulence
Wound healing
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Summary:The study of nanoparticles has gained popularity recently, particularly in biomedicine where metallic AgNPs were widely utilized for their therapeutic benefits. This current study focuses on biosynthesis of AgNPs using marine Streptomyces coelicoflavus MTK30, detailing their characterization, in-vitro, in-vivo and in-silico properties. UV-Vis spectrum exhibited a surface plasmon resonance peak at 441.24 nm, while the FT-IR spectrum O-H stretching at 3416.99 cm −1 showed the stabilization of AgNPs. XRD and SEM revealed the clear, crystalline, spherical shaped and their width was recorded as 98.4 nm with a PdI of 0.229 during DLS analysis. At 40 µl/ml concentration, showed antibacterial activity against clinical pathogens and significant scavenging against DPPH and MCF-7 with IC 50 values of 56.50 and 44.83 µg/ml. In-vivo studies using Swiss albino mice proclaimed that at 10 mg concentration is more effective in wound healing. Molecular docking studies elucidated the anticancer interaction with the breast cancer virulence protein (BRCA1 and C-erbB2) as well as various pathogenic bacteria (SpA, Ag43, esp, and aglD). This research demonstrates the feasibility of utilizing marine Streptomyces coelicoflavus MTK30 to produce biogenic AgNPs with significant antimicrobial, antioxidant, anticancer, wound healing and in-silico modelling revealed that well conserved binding region for drug development approaches. Graphical Abstract Highlights Biosynthesized AgNPs using marine Streptomyces coelicoflavus – MTK30 and characterized. IC 50 value of 44.83 µg/ml against MCF7 cancer cells showed its anti-cancer potential. In-vivo studies in Swiss albino mice models proclaimed that at 10 mg of AgNPs completely healed in 12 days. Molecular docking study of AgNPs showed good interaction in cancer virulence protein and bacterial virulence protein.
ISSN:0928-0707
1573-4846
DOI:10.1007/s10971-024-06441-6