Hyperpolarization of 15N‐Pyridinium by Using Parahydrogen Enables Access to Reactive Oxygen Sensors and Pilot In Vivo Studies

Magnetic resonance with hyperpolarized contrast agents is one of the most powerful and noninvasive imaging platforms capable for investigating in vivo metabolism. While most of the utilized hyperpolarized agents are based on 13C nuclei, a milestone advance in this area is the emergence of 15N hyperp...

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Bibliographic Details
Published in:Angewandte Chemie 2024-08, Vol.136 (33), p.n/a
Main Authors: Mei, Ruhuai, Fries, Lisa M., Hune, Theresa L. K., Santi, Maria Daniela, Rodriguez, Gonzalo Gabriel, Sternkopf, Sonja, Glöggler, Stefan
Format: Article
Language:English
Subjects:
15N-pyridinium
Biocompatibility
Contrast agents
Contrast media
Hydrogen peroxide
Hyperpolarization
In vivo methods and tests
Intracellular signalling
Magnetic resonance
Magnetic resonance imaging
Nitrogen isotopes
NMR
Organic solvents
Oxygen
Oxygen probes
Parahydrogen
PHIP
Probes
Pyridinium
Reactive oxygen species
Signal detection
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Summary:Magnetic resonance with hyperpolarized contrast agents is one of the most powerful and noninvasive imaging platforms capable for investigating in vivo metabolism. While most of the utilized hyperpolarized agents are based on 13C nuclei, a milestone advance in this area is the emergence of 15N hyperpolarized contrast agents. Currently, the reported 15N hyperpolarized agents mainly utilize the dissolution dynamic nuclear polarization (d‐DNP) protocol. The parahydrogen enhanced 15N probes have proven to be elusive and have been tested almost exclusively in organic solvents. Herein, we designed a reaction based reactive oxygen sensor 15N‐boronobenzyl‐2‐styrylpyridinium (15N‐BBSP) which can be hyperpolarized with para‐hydrogen. Reactive oxygen species plays a vital role as one of the essential intracellular signalling molecules. Disturbance of the H2O2 level usually represents a hallmark of pathophysiological conditions. This H2O2 probe exhibited rapid responsiveness toward H2O2 and offered spectrally resolvable chemical shifts. We also provide strategies to bring the newly developed probe from the organic reaction solution into a biocompatible injection buffer and demonstrate the feasibility of in vivo 15N signal detection. The present work manifests its great potential not only for reaction based reactive sensing probes but also promises to serve as a platform to develop other contrast agents. A 15N‐labelled tracer for magnetic resonance is introduced that is signal enhanced using parahydrogen and the feasibility for first pilot 15N in vivo studies is demonstrated.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.202403144